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Plant regeneration method of vinca from hairly root

A technique for regenerating plants and periwinkle flowers, applied in the biological field, can solve undiscovered problems and achieve the effect of solving the shortage of drug sources

Inactive Publication Date: 2007-10-03
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regeneration of complete plants from transgenic periwinkle hairy roots, screening and identification of transformed periwinkle plants with high content of terpene indole alkaloids can bring great power and benefits to theoretical research and production practice, and will eventually solve the problem of anti-tumor Scarcity of terpene indole alkaloid drugs provides high-quality new varieties of periwinkle, but no relevant reports have been found on the regeneration plant of periwinkle hairy root mentioned in the subject of the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Induction of Callus and Differentiation of Adventitious Buds in Vinca Hairy Roots

[0019] The explants of periwinkle hairy roots were cut into 2-3 cm long segments and cultured on callus induction medium, which was MS (Murashige and Skoog, 1962) + naphthaleneacetic acid (NAA ) 0.3mg / L+6-benzyladenine (6-BA) 2.0mg / L+3% sucrose+0.26% plant gel. After 10-20 days of light culture, the hairy roots can be covered with nodular callus, and the callus induction rate reaches 100%; the callus is inoculated on the adventitious bud induction medium for subculture 1- 2 generations, 20-25 days per generation, a large number of light green buds are produced on the surface of the callus, and the adventitious bud induction medium is MS + naphthaleneacetic acid (NAA) 0.3mg / L + 6-benzyladenine (6-BA) 2.0mg / L + 3% sucrose + 0.26% plant gel; select the callus containing green buds and continue to subculture on the above medium. After about 20 days of cultivation, the green buds will gradua...

Embodiment 2

[0022] Rooting of adventitious buds of periwinkle

[0023] The adventitious buds differentiated in Example 1 were cut and cultured in a rooting medium, the rooting medium was 1 / 2MS+3% sucrose+0.26% phytogel, the culture temperature was 25±1°C, and the light was 16 hours a day . After 8-10 days of cultivation, white root processes visible to the naked eye appear at the base of the adventitious buds. After 20 days of light cultivation, 5-10 adventitious roots can be differentiated. The differentiation rate of adventitious roots is 100%. After 30 days, 5 - In vitro plantlets 6 cm high with 3-5 pairs of leaves.

Embodiment 3

[0025] Rapid Propagation of Periwinkle Regenerated Plants

[0026] The vigorously growing periwinkle regenerated plants in Example 2 were selected and cultured in the rapid propagation medium by means of cutting and multiplication to form a periwinkle regenerated plant strain. The rapid propagation medium is MS medium + 3% sucrose + 0.26% phytogel without adding any plant growth regulating substances, the culture temperature is 25±1°C, and the light is 16 hours a day.

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Abstract

The present invention is method of regenerating vinca hairy root plant in biotechnology. The method of the present invention includes cutting sections of vinca hairy root explant, setting on callus tissue culture medium and lighting culturing to form callus, inoculating to adventitious bud culture medium to form bud, further lighting culturing to form adventitious bud, cutting adventitious bud and transferring to rooting culture medium to produce adventitious root, further culturing of the regenerated complete plant onto fast propagation culture medium, selecting plant and transplanting to flower pot with transplanting culture medium to obtain normally growing vinca plant via domestication. The present invention screens out proper culture medium, and has vinca hairy root adventitious bud differentiating rate up to 80 %, adventitious bud rooting rate up to 100 % and regenerated plant transplanting survival rate up to 100 %.

Description

technical field [0001] The invention relates to a method in the field of biotechnology, in particular to a method for regenerating plants from periwinkle hairy roots. Background technique [0002] Vinca (Catharanthus roseus) is a plant of the genus Vinca in the family Apocynaceae. At present, more than 100 terpene indole alkaloids have been isolated from the whole plant of Vinca, such as Vinblastine (Vinblastine) and Vincristine ( Vincristine), Ajmalicine, Serpentine, Vindoline, Catharanthine, etc. Most terpene indole alkaloids are widely used in modern medicine due to their biological activity. For example, the famous antineoplastic drugs vinblastine and vincristine are currently the most widely used natural plant antineoplastic drugs, and their sulfates have been Widely used clinically. Vinblastine and vincristine are currently the anticancer terpene indole alkaloids with the greatest and most widely used medicinal value in vinca. They are mainly extracted from natural ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 唐克轩龚一富苗志奇孙小芬
Owner SHANGHAI JIAO TONG UNIV
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