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Total triterpenoid extract fine thim asterium sponge and its preparation process and application thereof

A technology of astral sponge and total triterpenes, applied in the field of medicine, can solve problems such as no reports of anti-tumor activity, etc.

Inactive Publication Date: 2008-03-05
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of these compounds have certain cytotoxic activity, but no anti-tumor activity has been reported

Method used

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  • Total triterpenoid extract fine thim asterium sponge and its preparation process and application thereof
  • Total triterpenoid extract fine thim asterium sponge and its preparation process and application thereof
  • Total triterpenoid extract fine thim asterium sponge and its preparation process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 extract of the present invention

[0029] Take 10 kg of dry thin astral sponge, pulverize, extract by percolation with 75% ethanol with a volume / weight ratio of 6 times, recover ethanol under reduced pressure, add dichloromethane for extraction, recover dichloromethane under reduced pressure, and obtain the total extract , the total extract is dissolved in 85% methanol, extracted with petroleum ether, discarding the petroleum ether layer, adjusting the concentration of methanol to 65%, extracting with carbon tetrachloride, reclaiming carbon tetrachloride, and concentrating the gained ointment to dryness, Pass through Sephandex LH20 gel column, elute with dichloromethane / methanol whose mobile phase is 1:1 (v / v), collect the light yellow part, recover the solvent and concentrate to dryness, pass through 200-300 mesh normal phase silica gel column, and Elute with petroleum ether / ethyl acetate 15:1 (v / v), discard the eluent, and then elute wi...

Embodiment 2

[0030] Embodiment 2 Preparation of the extract of the present invention

[0031]Take 10 kg of dry thin astral sponge, pulverize, extract by percolation with 65% ethanol with a volume / weight ratio of 7 times, recover ethanol under reduced pressure, add dichloromethane for extraction, recover dichloromethane under reduced pressure, and obtain the total extract , the total extract is dissolved in 95% methanol, extracted with petroleum ether, discarding the petroleum ether layer, adjusting the concentration of methanol to 75%, extracting with carbon tetrachloride, reclaiming carbon tetrachloride, and concentrating the gained ointment to dryness, Pass through a Sephandex LH20 gel column, elute with n-hexane / dichloromethane / methanol with a mobile phase of 10:10:1 (v / v), collect the light yellow part, recover the solvent and concentrate to dryness, pass through 300-400 mesh phase silica gel column, eluted with petroleum ether / acetone 15:1 (v / v), discarded the eluent, and then eluted ...

Embodiment 3

[0032] The preparation of embodiment 3 extracts of the present invention

[0033] Take 10 kg of dry thin astral sponge, pulverize it, extract by percolation with 90% ethanol with a volume / weight ratio of 8 times, recover ethanol under reduced pressure, add dichloromethane for extraction, recover dichloromethane under reduced pressure, and obtain the total extract , the total extract is dissolved in 90% methanol, extracted with petroleum ether, discarding the petroleum ether layer, adjusting the methanol concentration to 70%, extracting with carbon tetrachloride, reclaiming carbon tetrachloride, and concentrating the gained ointment to dryness, Pass through a Sephandex LH20 gel column, elute with n-hexane / dichloromethane / methanol with a mobile phase of 4:5:1 (v / v), collect the light yellow part, recover the solvent and concentrate to dryness, pass through 200-300 mesh phase silica gel column, eluting with petroleum ether / ethyl acetate 10:1 (v / v), discarding the eluent, and then...

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Abstract

The invention relates to medication technology field that is zephyr asterism sponge triterpene extractive and the manufacturing method and the anti-tumor usage method. The triterpene compounding would be contained 50-90% in the extractive. It has been proved that has obvious anti-tumor activity.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a total triterpene extract in a thin astral sponge, a preparation method thereof and an antitumor application. Background technique [0002] The thin star sponge (Stelletta tenuis Lindgren) belongs to the class Demospongiae, the order Astrophorida, and the animal of the genus Stelletta in the family Stellettidae. This kind of sponge is widely distributed in the South China Sea, and is easy to collect in large numbers, and generally grows on exposed rocks on the seabed. The applicant extracted the initial extract from the thin astral sponge collected from the South China Sea, and found that it has cytotoxic activity, and then isolated twelve isomalabaricane (isomalabaricane) type triterpenoids, which are : stellettinA, B, C, D, E, H, geoditinA, B, isogeoditinA, B, 22, 23-dihydrostellettin B and rhabdastrellic acid, their chemical structures are as follows: [0003] [0004] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07B63/00C07D309/30C07C51/42A61K35/56A61K31/366A61P35/00A61K35/655
Inventor 林厚文王增蕾刘皋林陈万生
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY