Method for measuring ginger utilizing 6-gingerol oxime and 6-gingerol in products thereof

Abstract

The present invention relates to a method that can be used for qualitatively determing 6-gingeroloxime formed by means of oxime-removing reaction of 6-gingeroloxime and uses 6-gingeroloxime as internal standard of determination to determine gingerol content in fresh ginger and its products.

Description

technical field

[0001] The invention relates to a method for determining active ingredients in ginger, which is a medicinal and edible material, and its products. Background technique

[0002] Ginger (Zingiber officinal Roscoe) is not only a commonly used vegetable and condiment, but also a commonly used Chinese medicinal material (including fresh ginger juice, ginger peel, and processed ginger and ginger charcoal, etc. are commonly used drugs in traditional Chinese medicine). Xinjiang guards against all evils, and has the effects of expressing, expelling cold, relieving vomiting, and resolving phlegm. Because it is "vegetable, harmonious, fruity, and medicinal, its benefits are extensive", "Shenlong Materia Medica" listed it as the top grade, and the ginger liquid extract and ginger tincture recorded in the Mainland Pharmacopoeia and Taiwan Pharmacopoeia are still available. It is a widely used medicine for stomach problems. There are proverbs in our country that "eating ...

Examples

Embodiment 1

[0032] Embodiment one: the mensuration of 6-gingerol in fresh ginger

[0033] 1. Sample processing

[0034] Wash the fresh ginger, cut it into strips, and beat it in a beater. Weigh 1.000 g of ginger pulp, use 2 volumes of acetonitrile and 3 volumes of 2% acetic acid mixture, and dilute it to 10 ml to prepare a sample solution of 0.200 g of fresh ginger / ml. Put it in an ultrasonic homogenizer to oscillate and mix evenly, centrifuge at 3000 rpm, take the supernatant and filter it with a 0.22 μm nylon filter membrane. The filtrate can be used as a sample for the determination of 6-gingerol in fresh ginger. The injection volume was 2 microliters.

[0035] Add 5 μg of 6-gingerol oxime as an internal standard to the prepared samples.

[0036] 2. Conditions for determination by high pressure liquid chromatography

[0037] The injection volume at the time of measurement was 2 microliters. Mobile phase: a mixture of 2 volumes of acetonitrile and 3 volumes of 2% acetic acid; flow...

Embodiment 2

[0042] Embodiment two: the mensuration of 6-gingerol in ginger oleoresin

[0043] 1. Sample processing

[0044]Weigh 0.1000 g of ginger oleoresin, dilute to 5 ml with 2 volumes of acetonitrile and 3 volumes of 2% acetic acid mixed solution to prepare a sample solution containing 0.02 g of ginger oleoresin per ml. Put it in an ultrasonic homogenizer to oscillate and mix well, centrifuge at 10,000 rpm, and filter the supernatant with a 0.22 μm nylon filter membrane. The filtrate can be used as a sample for the determination of 6-gingerol in fresh ginger.

[0045] 2, the condition that high-pressure liquid chromatograph measures: with embodiment one.

[0046] 3, The qualitative of 6-gingerol: with embodiment one.

[0047] 4. Calculation of 6-gingerol content in ginger and its products

[0048] According to formula ①, the content of 6-gingerol is 4.01±0.18% (n=3).

Embodiment 3

[0049] The mensuration of 6-gingerol in the dried ginger of embodiment three

[0050] 1. Sample processing

[0051] Weigh 1.000 g of dried ginger, use 10 ml of a mixture of 2 volumes of acetonitrile and 3 volumes of 2% acetic acid, oscillate and mix in an ultrasonic homogenizer, centrifuge at 3000-10000 speeds, take the supernatant and filter it with 0.22 μm nylon Membrane filtration. Use 2 volumes of acetonitrile and 3 volumes of 2% acetic acid to dilute to 100 ml. Add 500 µg of 6-gingerol oxime as an internal standard to the prepared samples.

[0052] 2, the condition that high-pressure liquid chromatograph measures: with embodiment one.

[0053] 3, The qualitative of 6-gingerol: with embodiment one.

[0054] 4. Calculation of 6-gingerol content in ginger and its products

[0055] Calculate the content of 6-gingerol according to ① formula: 0.22±0.016% (n=3)

technical field

[0001] The invention relates to a method for determining active ingredients in ginger, which is a medicinal and edible material, and its products. Background technique

[0002] Ginger (Zingiber officinal Roscoe) is not only a commonly used vegetable and condiment, but also a commonly used Chinese medicinal material (including fresh ginger juice, ginger peel, and processed ginger and ginger charcoal, etc. are commonly used drugs in traditional Chinese medicine). Xinjiang guards against all evils, and has the effects of expressing, expelling cold, relieving vomiting, and resolving phlegm. Because it is "vegetable, harmonious, fruity, and medicinal, its benefits are extensive", "Shenlong Materia Medica" listed it as the top grade, and the ginger liquid extract and ginger tincture recorded in the Mainland Pharmacopoeia and Taiwan Pharmacopoeia are still available. It is a widely used medicine for stomach problems. There are proverbs in our country that "eating ...

Examples

Embodiment 1

[0032] Embodiment one: the mensuration of 6-gingerol in fresh ginger

[0033] 1. Sample processing

[0034] Wash the fresh ginger, cut it into strips, and beat it in a beater. Weigh 1.000 g of ginger pulp, use 2 volumes of acetonitrile and 3 volumes of 2% acetic acid mixture, and dilute it to 10 ml to prepare a sample solution of 0.200 g of fresh ginger / ml. Put it in an ultrasonic homogenizer to oscillate and mix evenly, centrifuge at 3000 rpm, take the supernatant and filter it with a 0.22 μm nylon filter membrane. The filtrate can be used as a sample for the determination of 6-gingerol in fresh ginger. The injection volume was 2 microliters.

[0035] Add 5 μg of 6-gingerol oxime as an internal standard to the prepared samples.

[0036] 2. Conditions for determination by high pressure liquid chromatography

[0037] The injection volume at the time of measurement was 2 microliters. Mobile phase: a mixture of 2 volumes of acetonitrile and 3 volumes of 2% acetic acid; flow...

Embodiment 2

[0042] Embodiment two: the mensuration of 6-gingerol in ginger oleoresin

[0043] 1. Sample processing

[0044]Weigh 0.1000 g of ginger oleoresin, dilute to 5 ml with 2 volumes of acetonitrile and 3 volumes of 2% acetic acid mixed solution to prepare a sample solution containing 0.02 g of ginger oleoresin per ml. Put it in an ultrasonic homogenizer to oscillate and mix well, centrifuge at 10,000 rpm, and filter the supernatant with a 0.22 μm nylon filter membrane. The filtrate can be used as a sample for the determination of 6-gingerol in fresh ginger.

[0045] 2, the condition that high-pressure liquid chromatograph measures: with embodiment one.

[0046] 3, The qualitative of 6-gingerol: with embodiment one.

[0047] 4. Calculation of 6-gingerol content in ginger and its products

[0048] According to formula ①, the content of 6-gingerol is 4.01±0.18% (n=3).

Embodiment 3

[0049] The mensuration of 6-gingerol in the dried ginger of embodiment three

[0050] 1. Sample processing

[0051] Weigh 1.000 g of dried ginger, use 10 ml of a mixture of 2 volumes of acetonitrile and 3 volumes of 2% acetic acid, oscillate and mix in an ultrasonic homogenizer, centrifuge at 3000-10000 speeds, take the supernatant and filter it with 0.22 μm nylon Membrane filtration. Use 2 volumes of acetonitrile and 3 volumes of 2% acetic acid to dilute to 100 ml. Add 500 µg of 6-gingerol oxime as an internal standard to the prepared samples.

[0052] 2, the condition that high-pressure liquid chromatograph measures: with embodiment one.

[0053] 3, The qualitative of 6-gingerol: with embodiment one.

[0054] 4. Calculation of 6-gingerol content in ginger and its products

[0055] Calculate the content of 6-gingerol according to ① formula: 0.22±0.016% (n=3)

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