46 results about "6-gingerol" patented technology

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

An aspect of the present invention relates to compositions comprising a gingerol, for example, compositions comprising gingerol in an amount greater than about 2% by weight. In some aspects of the invention, the composition comprises 6-gingerol, 8-gingerol, 10-gingerol, 6-shagaol, or combinations thereof. Another aspect of the invention relates to a method for extracting a ginger species comprising, sequentially extracting a ginger species plant material to yield an essential oil fraction, a gingerol fraction, a phenolic fraction, and a polysaccharide fraction, wherein the essential oil and gingerol fractions are derived by extracting plant feedstock material by supercritical carbon dioxide extraction, the phenolic fraction is extracted from the plant feedstock material or from the remainder of the essential oil and gingerol extractions by hydroalcoholic extraction, and the polysaccharide fraction is derived by water extraction of the remainder of the phenolic extraction.

The invention discloses a separating method of 6-gingerol, which is characterized by the following: adopting multiple chromatographic ways to separate effective component 6-gingerol in the traditional Chinese medicine ginger; accelerating the separating course; simplifying the operation; improving the quality and production.

The invention provides a fingerprint spectrum detecting method of a common goldenrop particle compound preparation, containing radix bupleuri, scutellaria baicalensis, codonopsis pilosula, ginger and licorice root. The method comprises firstly preparing baicalein, baicalin, Codonopsis pilosula Acetylene glycosides, saikoside C, wogonin, 6-gingerol, ammonium glycyrrhetate, saikoside A and saikoside B2 into a reference substance mixed solvent, secondly, extracting a common goldenrop particle compound preparation to be tested and extracting to prepare a test solvent, thirdly, sucking the reference substance mixed solvent and injecting into a high performance liquid chromatograph, and recording a contrast chromatogram, next, sucking the test solvent, injecting into the high performance liquid chromatograph, and recording a test chromatogram, and then comparing the contrast chromatogram and the test chromatogram. The detection method establishes a complete fingerprint spectrum detection method, the common goldenrop particle compound preparation is comprehensively detected, and quality of the common goldenrop particle compound preparation is more comprehensively and effectively controlled.

The technological process of separating 6-gingerol from ginger includes the steps of: preparing ginger oleoresin, chromatographic separation with silica gel column, preparing coarse gingerol, liquid phase chromatographic separation, eliminating solvent, preparing pure 6-gingerol product, etc. The present invention has high 6-gingerol purity, simple operation, simple technological process and high separation efficiency.

This invention is to provide multiple specific inhibitors of cytochrome P450 isozyme CYP2C9. These inhibitors can be derived from any combinations with the following compounds including: Tamarixetin, Formononetin, isoliquritigenin, Phloretin, luteolin, Quercitrin, quercetin, myricetin, Wongonin, Puerarin, Genistein, Nordihydroguaiaretic acid, Narigenin, Capillarisin, Chrysin, Fisefin, eriodictyol, 6-Gingerol, Isorhamneti, isoquercitrin, Morin, (+)-Taxifolin, isovitexin, 3-Phenylpropyl Acetate, Oleanolic acid, ursolic acid, β-Myrcene, cinnamic acid, Luteolin-7-Glucoside, Liquiritin, (+)Limonene, Homoorientin, Swertiamarin, Embelin, Daidzein, Poncirin, (−)-Epicatechin, ergosterol. These natural products can be used to enhance the bioavailability of therapeutic agents (drugs).

The invention relates to a biochemical engineering separating method of natural active products, and especially relates to a method for simultaneously extracting and separating ginger essential oil and ginger oil resin enriched in gingerol in ginger by a supercritical carbon dioxide fluid. The method is characterized in that the pressure and temperature of an extraction kettle is changed, the ginger essential oil containing little 6-gingerol and 6-gingerol-enriched ginger oil resin with large appearance and property difference can be obtained in a separation kettle in different extraction and separation phases. The method uses a separation kettle, the process flow is simple, two important extraction products such as ginger essential oil and ginger oil resin in ginger can be used in the fields of food, cosmetic, medicine and the like and clearly obtained at a time, the investment for equipments and workshops is small, and the large scale production can be carried out.

The present invention relates to a method that can be used for qualitatively determing 6-gingeroloxime formed by means of oxime-removing reaction of 6-gingeroloxime and uses 6-gingeroloxime as internal standard of determination to determine gingerol content in fresh ginger and its products.

The invention relates to a ginger phenols extract preparation, in particular to a ginger phenols extract soft capsule and a preparation method thereof. The content of the soft capsule comprises 3-10 parts of ginger phenols extract and 40-60 parts of oleaginous base by weight part. Preferably, the content of the soft capsule comprises 6 parts of ginger phenols extract and 54 parts of corn oil by weight. The extraction method of the ginger phenols extract in the soft capsule is supercritical carbon dioxide extraction method which has the advantages of high extraction rate and high content of 6-gingerol. Meantime, the ginger phenols extract soft capsule has good stability.

The invention discloses a method for preparing 6-gingerol and 8-gingerol from ginger and an ultrafiltration device thereof and belongs to the technical field of the preparation method of natural active products. The method disclosed by the invention sequentially comprises the steps of treating the raw material of the ginger, mixing the homogenate, removing the impurities to obtain a primary extractive, ultra-filtrating to obtain a primary product, and nano-filtrating to obtain a pure product. The ultrafiltration device comprises at least one shell and a pipe unit communicated with the inner cavity of the shell; a closed tubular ultra-filtration is arranged in the inner cavity of the shell; two pipes communicated with the inner cavity of the ultra-filtration are arranged on the upper end part of the ultra-filtration; and an ultrafiltration membrane capable of allowing materials to be filtered to enter a hollow cavity between the ultra-filtration and the shell from the inner cavity of the ultra-filtration is arranged on the tubular wall of the ultra-filtration. Compared with the prior art, the invention has the substantive characteristics and the remarkable progresses of easily-controlled preparation process, convenient and simple operational process, high production efficiency, suitability for large-scale of factory production and capability of improving the level of the health of people.

The invention relates to the fields of separation and purification and provides a method for separating and purifying 6-gingerol by reduced pressure column chromatography and a production method of the 6-gingerol. The method for separating and purifying the 6-gingerol by the reduced pressure column chromatography comprises the following steps: performing gradient elution on a raw material containing the 6-gingerol through a first mixed solvent with N-hexane-ethyl acetate as a mobile phase in different proportions to obtain a 6-gingerol crude product; and performing gradient elution on the 6-gingerol crude product in a second reduced pressure column through a second mixed solvent with N-hexane-ethyl acetate as a mobile phase in different proportions to obtain a 6-gingerol purified substance. The method for separating and purifying the 6-gingerol by the reduced pressure column chromatography and the production method of the 6-gingerol, provided by the invention, have the benefits that the 6-gingerol is separated and purified by utilizing a two-step reduced pressure gradient elution method; through the reduced pressure operation, the operation is easier to control, and the elution time is short; meanwhile, the N-hexane-ethyl acetate mixed in different proportions is adopted as the mobile phase, the mobile phase is few in types, the consumption is less, and the recovery is easy.

The invention discloses an effective part of a small Pinellia ternate decoction. The effective part has the following physical and chemical properties: the effective part is a yellow oily substance, and the effective part which has certain volatility can be dissolved in hot water and ethanol; and the effective part which mainly contains gingerol and shogaol components concretely contains 40-50% of 6-gingerol and 12-20% of 6-shogaol. The invention also discloses a preparation method and an application of the effective part of the small Pinellia ternate decoction. The fat-soluble effective partextracted from the small Pinellia ternate decoction has the following obvious pharmacological drug effects: 1, the vomiting prevention effect is obvious, results of experiments of pigeon vomiting caused by copper sulfate and a chemotherapeutic cis-platinum as experiment models show that the fat-soluble part has a substantial vomiting prevention activity and can obviously reduce the vomiting frequency of pigeons; and 2, the effective part has a normal mouse stomach evacuation inhibition effect.

The invention discloses a method for continuously extracting and purifying ginger polyphenol. According to the method, firstly, the ginger polyphenol is extracted in a combined way by an enzyme-ultrasound method; after the purification by an ultra-pure method, AB-8 macroporous resin adsorption is performed; then, a polyamide column, a silicon gel column and a Sephadex LH-20 gel column are continuously used for chromatography purification; the sample is subjected to freeze drying to obtain a purified sample. The maximum absorption wavelength of the polyphenol obtained from the ginger polyphenolpurified sample prepared by the method through UV scanning and CG-MS analysis is 281 nm; the result of the CG-MS analysis proves that the purified materials mainly contains 66.38 percent of 6-gingerol, 3.12 percent of 8-gingerol, 2.06 percent of Z-6-shogaol, 1.93 percent of 8-shogaol, 2.53 percent of citral and 9.35 percent of capraldehyde (heating degrading products of shogaol). The purity of the ginger polyphenol prepared by the method is as high as 85.37 percent.

The invention relates to a natural composite antioxidant and an antioxidant method thereof for inhibiting marine fish lipid oxidation. The natural composite antioxidant is prepared by dissolving 6-gingerol, apple polyphenol, a purple bellis perennis extract and chitosan in glacial acetic acid, and stirring the materials evenly. The antioxidant method includes: dipping marine fish slices in the natural composite antioxidant, then taking the slices out and conducting draining, loading the slices into a polyethylene bag and performing sealing, then storing the product at 4+ / -1DEG C. The method can significantly retard lipid oxidation of marine fish slices. All components of the natural composite antioxidant provided by the invention are natural products, have synergistic effect, and are safe, efficient, and free of toxic and side effect. The method is easy to operate, and is widely applicable to preservation and freshness keeping of various marine fish.

The invention discloses a method for simultaneously detecting the content of four components in Huangqi Guizhi Wuwu Granule. The four components are paeoniflorin, calycosin-7-glucoside, cinnamaldehydeand 6-gingerol. The method comprises the steps of extracting a to-be-detected sample by using an extraction solvent: 30-80% methanol, filtering extract and directly detecting by using a high-performance liquid chromatograph. Through the method disclosed by the invention, the content of the four components in the Huangzhi Guizhi Wuwu Granule can be simultaneously detected and the method has the advantages of high efficiency and accuracy and the like, so that the quality of the Huangqi Guizhi Wuwu Granule is more controllable.

The invention belongs to the technical field of chemical engineering, and particularly relates to a separating and extracting method for 6-gingerol in fresh ginger. The silica gel column chromatography ascending development method is used so that 6-gingerol can be individually separated from a silica gel chromatography column in quantity. The problem that due to the fact that 6-gingerol is easy to decompose when heated, 6-gingerol is difficult to separate is solved. The purity of 6-gingerol obtained through the method is 85% or more. Meanwhile, the method is convenient, fast, low in cost, easy to implement and suitable for large-scale application and popularization.

The invention discloses a quality control method for dried ginger. The method comprises the following steps of: determining the content of 4 ginger phenolic chemical components including 6-gingerol, 8-gingerol, 6-shogaol and 10 gingerol, and 2 volatile oil chemical components including alpha-curcumene and (E)-beta-farnesene in the dried ginger medicinal materials by adopting an HPLC-PDA method; and taking the total amount of the ginger phenolic components, the total amount of the volatile oil components, or the total amount of the ginger phenolic components and the volatile oil components as an evaluation index, which is used for formulating a standard for evaluating the quality of the dried ginger medicinal material and controlling the quality. The method is capable of comprehensively, objectively and effectively evaluating the quality of the dried ginger medicinal material by simultaneously detecting two types of the active ingredients of the dried ginger medicinal material and analyzing the fingerprint spectrum and comparing with a single analysis of one or a class of chemical components, and is used for the quality control thereof, and the method is simple and convenient to operate, good in stability and easy to be popularized.

The invention relates to a natural medicine composition used for treating cardiovascular diseases. The natural medicine composition comprises aconitine, liquiritin and 6-gingerol and is prepared into oral preparations, such as hard capsules, soft capsules, tablets, granules, powder, pills, tea bags and medicinal liquor by utilizing synergistic effects of the components. The composition can achieve the effects of treating rats with heart failures caused by myocardial infarction, increasing the ejection fractions and fractional shortening of the rats, obviously improving cardiomyocyte morphologies, reducing the degree of cardiac collagen fibrosis and reducing the contents of aldosterone and atrial natriuretic peptide in serums, and has the function of treating cardiovascular diseases. The composition has definite pharmacodynamic effects, is rigorous in quality control and small in taking dosage and is convenient to take.

The invention relates to the field of separation and purification, and provides a separation and purification method and a production method of 6-gingerol. The separation and purification method of the 6-gingerol comprises the following steps of: dissolving a raw material containing the 6-gingerol into a first organic solvent, and then carrying out liquid-liquid extraction separation on the firstorganic solvent in which the raw material containing the 6-gingerol is dissolved by using a second organic solvent which is insoluble in the first organic solvent. The production method of the 6-gingerol comprises an extracting method of the 6-gingerol and the separation and purification method of the 6-gingerol. According to the separation and purification method of the 6-gingerol, provided by the embodiment of the invention, the 6-gingerol can be quickly separated and purified through liquid-liquid extraction, e consumed time is short, the organic solvents can be recycled, a separation and purification process is simple, the required equipment is conventional equipment, the yield is high, and large-batch production is easy. According to the production method of the 6-gingerol, provided by the embodiment of the invention, the 6-gingerol obtained through extraction, separation and purification has the advantages of high content, higher yield, shorter consumed time and less organic solvent consumption.

The invention provides a simple, convenient and efficient process for synthesizing 6-gingerol, 8-gingerol and 10-gingerol. Deprotonation and straight-chain fatty aldehyde condensation of zingiberone are performed in an inert solvent, so that the crude product of gingerol are obtained. The crude product is subjected to posttreatment at a low temperature and purified through silica gel column chromatography so that 6-gingerol, 8-gingerol or 10-gingerol having the purity exceeding 95% can be obtained. Besides, the yield exceeds 70%.

The invention relates to a preparation method of 6-gingerol. The preparation method comprises the following steps: (1), pretreating: adding an ethanol solution into ginger resin, dissolving, and thenadding water to adjust the volume fraction of ethanol to 50-60% while homogenizing to obtain a ginger resin-ethanol mixed solution; (2), cooling and removing impurities: cooling the ginger resin-ethanol mixed solution in the step (1) till a precipitate is separated out, then raising the temperature to the room temperature, waiting till the precipitate is melted into an oily substance, and then separating to remove the oily substance to obtain an ethanol solution phase; (3), performing liquid-liquid extraction: adjusting the volume fraction of the ethanol in the ethanol solution phase in the step (2) to 70-90%, performing the liquid-liquid extraction by using n-hexane, collecting an ethanol phase, concentrating and removing water to obtain crude 6-gingerol. The content of the 6-gingerol inthe crude 6-gingerol prepared by the preparation method is relatively high, so that obtainment of the high-purity 6-gingerol through purification is facilitated, the yield of the 6-gingerol is increased and the preparation cost is reduced.

The invention discloses a method for extracting and separating 6-gingerol from ginger. The method comprises the following operation steps: solvent extracting, low-pressure silica gel column chromatography separation, high performance liquid chromatography purification and recrystallization. The yield of the 6-gingerol prepared by the method is high; the prepared 6-gingerol is high in biological activity and high in purity, and can be directly used for preparing drugs; the 6-gingerol can be stored in cryoprotectant for a long time; and the biological activity of the 6-gingerol is not reduced.

The invention relates to a quality detection method of a gingerol type extract and soft capsules thereof. The quality detection method specifically comprises trait inspection, identification, content determination and the like, wherein the content determination comprises the determination of total phenols and the determination of 6-gingerol, the determination of the total phenols adopts ultraviolet spectrophotometry, and the determination of the 6-gingerol adopts high performance liquid chromatography. According to the quality detection method disclosed by the invention, by improving the content determination method, the production quality level of an enterprise can be ensured, and the efficacy can be ensured.

The technological process of separating 6-gingerol from ginger includes the steps of: preparing ginger oleoresin, chromatographic separation with silica gel column, preparing coarse gingerol, liquid phase chromatographic separation, eliminating solvent, preparing pure 6-gingerol product, etc. The present invention has high 6-gingerol purity, simple operation, simple technological process and high separation efficiency.

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin,β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The present invention features a novel, low side-effect pharmaceutical compound complex, comprising the pharmaceutically effective dose of isoniazid (INH) and pharmaceutically effective dose of one of the following compounds. Said compound was selected from the following groups of compounds: Nordihydroguaiaretic acid, Trans-Cinnamaldehyde, Daidzein, Isovitexin, Kaempferol, disulfuram, β-Myrcene, Quercetin, (−)-Epigallocatechin-3-gallate, (+)-Limonene, Myricetin, Quercitrin, Luteolin-7-Glucoside, Morin, Neohesperidin, Hesperidin, Capillarisin, (−)-Epigallocatechin, Luteolin, Hyperoside, Ethyl Myristate, Tamarixetin, Phloretin, Baicalein, Rutin, Baicalin, Apigenin, Naringenin, Hesperetin, (+)-Epicatechin, (−)-Epicatechin-3-gallat, Isoliquritigenin, Silybin, Vitexin, Genistein, Isorhamnetin, gallic acid, Diosmin, 6-Gingerol, (+)-Taxifolin, Wongonin, Protocatechuic acid, (+)-Catechin, β-naphthoflavone, Embelin, Trans-Cinnamic acid, (−)-Epicatechin, Phloridzin, Puerarin, Umbelliferone, Brij 58, Brij 76, Brij 35, Tween 20, Tween 80, Tween 40, PEG 2000, PEG 400, Pluornic F68, and PEG 4000. The novel, low side-effect compound complex which contains pharmaceutically effective doses of isoniazid (INH), disulfuram (DSF) and / or a third compound, bis-nitrophenyl phosphate (BNPP) can reduce isoniazid (INH)-induced side effects, e.g. hepatotoxicity, etc.

The invention relates to a composition having effect of relieving pain. According to the composition, total gingerol is used as an active component, pharmaceutically acceptable auxiliary materials orauxiliary components are added, and a preparation is prepared, wherein the total content of 6-gingerol, 8-gingerol and 10-gingerol in the total gingerol is greater than 50%. The composition having theeffect of relieving pain is good in transdermal absorption effects, notable in medicine effects, convenient to use, and free from stimulation to stomach and intestines. A preparation method of the active component namely the total gingerol is simple in operation steps, the used raw materials are low in price, the obtained total gingerol is high in quality, and the total gingerol is free from special smell of fresh ginger, and is suitable for being used as an active component of an external preparation.