Orange peel soup fermented beverage and preparation method thereof
A technology of fermented beverage and orange peel soup, which is applied in the field of orange peel soup fermented beverage and its preparation, can solve the problem of less traditional Chinese medicine, and achieve the effect of facilitating absorption and increasing flavor substances
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Embodiment 1
[0033] (1) Preparation of reference substance solution
[0034]Accurately weigh 3.336mg, 0.950mg, 1.000mg, and 0.550mg of standard substances of nobiletin, hesperidin, 6-gingerol, and 8-gingerol into four 10mL volumetric flasks, add anhydrous methanol to dissolve, and constant volume to 10mL, shake well to prepare nobiletin, hesperidin, 6-gingerol, 8-gingerol standard solution.
[0035] Take 1 mL of each of the above standard solutions in a 10 mL volumetric flask, and add methanol to constant volume to 10 mL to prepare a mixed standard solution containing nobietin, hesperidin, 6-gingerol, and 8-gingerol.
[0036] (2) HPLC chromatographic conditions
[0037] The chromatographic column is: Agilent-ZORBAX SB-C18 chromatographic column (250mm×4.6mm, 5.0μm), the mobile phase is 0.1% formic acid water (A) ~ methanol (B), gradient elution, elution program: 0 ~ 5min, 10% B; 5 ~ 10min, 15% ~ 18% B; 10 ~ 15min, 18% ~ 20% B; 15 ~ 20min, 20% ~ 22% B; 20 ~ 25min, 22% B; 25 ~ 30min, 22% ...
Embodiment 2
[0044] Ginger and tangerine peel separate fermentation embodiment
[0045] (1) Ginger fermented liquid preparation:
[0046] Wash and slice the ginger, mix the ginger slices and white sugar evenly at a ratio of 7:3, add a compound probiotic agent with a weight ratio of 1‰, and place it in a constant temperature incubator at 30°C for cultivation, stirring every day 1 time, stir evenly and take samples on the 0th, 2nd, 4th, 9th, 14th, and 21st days of fermentation respectively (the sample solution obtained on the 0th day is after the addition of compound probiotics, immediately stir evenly and sample to obtain an unfermented sample solution), Store in a -20°C freezer for later use. Three fermentation broths were prepared in parallel as described above.
[0047] The main active ingredient in ginger is gingerol, which is also one of the important components of spicy substances, including 6-gingerol, 8-gingerol, 10-gingerol, 12-gingerol, etc., of which 6- Gingerol has the highes...
Embodiment 3
[0052] Orange peel soup fermented liquid preparation of the present invention:
[0053] Add ginger, tangerine peel, white sugar and water into a sterilized conical flask in a ratio of 2:1:3:5 (white sugar is added after dissolving in sterile water), mix well, and add 1‰ of Mix the probiotic agent, place it in a constant temperature incubator at 30°C for cultivation, stir once a day, stir evenly and take samples on the 0th, 2nd, 4th, 9th, 14th, and 21st days of fermentation respectively (the sample liquid obtained on the 0th day , after the compound probiotics are added, stir immediately and evenly sample to obtain unfermented sample liquid). Three fermentation broths were prepared in parallel as described above.
[0054] There is a significant difference in the change of the main active ingredients between the orange peel soup of probiotic fermented ginger and orange peel, and the single fermented ginger and orange peel. Depend on Figure 4 It can be seen that, except for 8...
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