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Method for extracting and separating 6-gingerol from ginger

A gingerol and ginger technology, applied in the field of ginger extract extraction, can solve the problems of unstable physical and chemical properties, loss of activity, and low purity of 6-gingerol, and achieve no reduction in biological activity, high biological activity, and high yield Effect

Inactive Publication Date: 2017-09-12
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

6-gingerol contains 8-hydroxyketone structure, its physical and chemical properties are unstable, and it is easily disturbed by external conditions and loses its activity
The 6-gingerol obtained by this method has a very low purity and is only suitable for applications in the fields of food and cosmetics

Method used

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  • Method for extracting and separating 6-gingerol from ginger
  • Method for extracting and separating 6-gingerol from ginger
  • Method for extracting and separating 6-gingerol from ginger

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting and separating 6-gingerol from ginger, carried out according to the following steps:

[0026] (1) Solvent extraction

[0027] Ginger (Zingeber officinle Rosc, produced in Laiwu, Shandong, collected in autumn) 300 grams, cut into pieces, add 2 times the amount of ethyl acetate, reflux in a water bath for 3 hours, pour out the ethyl acetate, and add 2 times the amount of ethyl acetate Extracted 3 times, combined the ethyl acetate extract, recovered ethyl acetate under reduced pressure, and obtained 2 grams of dark brown oily extract.

[0028] (2) Gingerol crude product is separated by low-pressure silica gel column chromatography

[0029] Use 200-300 mesh chromatography silica gel to dry-pack the column, add 5ml of ethyl acetate to dissolve the crude extract extracted in the previous step, mix it with 4g of silica gel, evaporate the ethyl acetate, install it on the top of the column, and add 3g of pure silica gel On it, elute with petroleum ether...

Embodiment 2

[0057] A method for extracting and separating 6-gingerol from ginger, carried out according to the following steps:

[0058] (1) Solvent extraction

[0059] Ginger (Zingeber officinle Rosc, produced in Laiwu, Shandong, collected in autumn) 300 grams, cut into pieces, add 2 times the amount of ethyl butyrate, reflux in a water bath for 3 hours, pour out the ethyl butyrate, and add 2 times the amount of diced The ethyl butyrate was extracted 3 times, the ethyl butyrate extract was combined, and the ethyl butyrate was recovered under reduced pressure to obtain 2 g of a dark brown oily extract.

[0060] (2) Gingerol crude product is separated by low-pressure silica gel column chromatography

[0061] Use 200-300 mesh chromatography silica gel to dry-pack the column, add 5ml of ethyl acetate to dissolve the crude extract extracted in the previous step, mix it with 4g of silica gel, evaporate the ethyl acetate, install it on the top of the column, and add 3g of pure silica gel On i...

Embodiment 3

[0077] The 6-gingerol product freezing liquid consists of the following components in parts by weight: 25 parts of vitamin C, 400 parts of ethanol, and 2 parts of water sedge extract.

[0078] The extraction method of the water sedge extract is as follows: freeze the fresh water sedge stalks in liquid nitrogen, take out and grind them into powder, add 3-8 times the weight of 70% ethanol aqueous solution to dissolve, reflux extraction 3 times, combine The filtrate was evaporated to dryness.

[0079]The 6-gingerol product prepared in Example 1 was mixed with the freezing solution at a ratio of 1:10, stored in a -4°C refrigerator, and the biological activity did not decrease within 1 year.

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Abstract

The invention discloses a method for extracting and separating 6-gingerol from ginger. The method comprises the following operation steps: solvent extracting, low-pressure silica gel column chromatography separation, high performance liquid chromatography purification and recrystallization. The yield of the 6-gingerol prepared by the method is high; the prepared 6-gingerol is high in biological activity and high in purity, and can be directly used for preparing drugs; the 6-gingerol can be stored in cryoprotectant for a long time; and the biological activity of the 6-gingerol is not reduced.

Description

technical field [0001] The present invention relates to a kind of extracting method of ginger extract, particularly a kind of method for extracting and separating 6-gingerol from ginger. Background technique [0002] There are a series of gingerol homologues in ginger. Due to their similar structure and similar properties, it is difficult to purify them into monomer components by conventional methods. The components separated on the low-pressure column chromatography column are identified as single spots by thin-layer chromatography. In fact, it is a family of compounds. After further separation by high performance liquid chromatography, 5 compounds are obtained, and one of the main components is 6-gingerol. Containing 8-hydroxyketone structure in 6-gingerol, its physical and chemical properties are unstable, and it is easily disturbed by external conditions and loses its activity. At present, the extraction method of 6-gingerol mostly adopts the method of chemical extracta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C45/78C07C49/255
CPCC07C45/78C07C49/255
Inventor 陈小明盘俊曾飞何福林
Owner HUNAN UNIV OF SCI & ENG
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