Method for continuously extracting and purifying ginger polyphenol
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A technology of ginger and combined extraction, used in agriculture and light industry, can solve the problems of high maintenance requirements, low extraction efficiency of phenolic substances, and large solvent consumption.
Inactive Publication Date: 2018-08-03
SHANDONG AGRICULTURAL UNIVERSITY
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These methods have their own advantages and disadvantages, but there are many disadvantages in single application, such as long time-consuming, high labor intensity, high energy consumption for pretreatment, large solvent consumption, serious damage to the structure of phenolic substances, and low extraction efficiency. The process is more expensive and requires higher maintenance
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experiment example
[0053] Experimental example: selection of process parameters
[0054] The raw material used in this experiment is Anqiu ginger provided by Shandong Datang Biotechnology Co., Ltd.
[0055] (1) Selection of enzymatic extraction process
[0056] ① Enzyme dosage selection
[0057] According to experiments, when the amount of cellulase added is between 0.5% and 1%, the yield of polyphenols increases with the increase of enzyme amount, and reaches the maximum value at 1%. After more than 1%, the yield basically remained the same, and the difference was not significant. Such as figure 1 shown. Therefore, the optimal amount is 1%.
[0058] ②Enzymolysis temperature selection
[0059] According to experiments, within 50°C, as the temperature increases, the yield of polyphenols increases gradually, and reaches the maximum at 50°C. After 50°C, the yield gradually decreased with the increase of temperature. Such as figure 2 shown. Therefore, the optimum enzymatic hydrolysis temp...
Embodiment
[0168] Embodiment: the preparation of ginger polyphenol
[0169] Add 1g cellulase according to every 100g shredded ginger, enzymolysis 50min at 35°C, collect the supernatant, add 2 times (mass: volume) 80% ethanol solvent to the remaining ginger slag, and ultrasonically extract twice under 400W power. After 1.5 hours, the supernatants were collected twice and combined, and the yield of ginger polyphenols extracted by the enzyme-ultrasonic method was 0.551%;
[0170] Adjust the concentration of ginger polyphenols in the supernatant extracted by enzyme-ultrasonic method to 0.5mg / mL, and perform ultrafiltration purification. The conditions are 30KD ultrafiltration membrane, 30°C, 0.15MPa pressure, and the membrane flux is 33.34mL *m -2 *s -1 , collect the ultra-filtered ginger polyphenol liquid less than 30KD;
[0171] The ultrafiltration ginger polyphenol solution was purified by the AB-8 macroporous resin adsorption method. The static adsorption conditions were: the optimal ...
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Abstract
The invention discloses a method for continuously extracting and purifying ginger polyphenol. According to the method, firstly, the ginger polyphenol is extracted in a combined way by an enzyme-ultrasound method; after the purification by an ultra-pure method, AB-8 macroporous resin adsorption is performed; then, a polyamide column, a silicon gel column and a Sephadex LH-20 gel column are continuously used for chromatography purification; the sample is subjected to freeze drying to obtain a purified sample. The maximum absorption wavelength of the polyphenol obtained from the ginger polyphenolpurified sample prepared by the method through UV scanning and CG-MS analysis is 281 nm; the result of the CG-MS analysis proves that the purified materials mainly contains 66.38 percent of 6-gingerol, 3.12 percent of 8-gingerol, 2.06 percent of Z-6-shogaol, 1.93 percent of 8-shogaol, 2.53 percent of citral and 9.35 percent of capraldehyde (heating degrading products of shogaol). The purity of the ginger polyphenol prepared by the method is as high as 85.37 percent.
Description
technical field [0001] The invention relates to a technique for jointly extracting multiple functional components of ginger, which belongs to the fields of light industry and agriculture. Background technique [0002] Ginger is not only a common cooking ingredient and spice seasoning in daily diet, but also a warming and tonic material commonly used in traditional Chinese medicine. Modern medical research shows that ginger has the functions of protecting the digestive system such as protecting the liver and gallbladder, invigorating the stomach and antiemetic, improving the body's immunity, promoting blood circulation, regulating the central nervous system, anti-inflammatory, antibacterial, and insecticidal; ginger extract can inhibit the activity of The generation of oxygen and the oxidation of linoleic acid can reduce serum and liver cholesterol, lower blood sugar, lower blood fat, anti-aging, anti-tumor, regulate body temperature and metabolism, and the research on its re...
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