Effective part of small Pinellia ternate decoction, preparation method thereof and application thereof

A technology of effective parts and Xiao Banxia Decoction, which is applied to the effective parts of Xiao Banxia Decoction and the field of preparation thereof, can solve the rare problems and the like, and achieve the effect of obvious pharmacological and pharmacodynamic effects.

Inactive Publication Date: 2012-07-11
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methods and related preparations for isolating effective parts and active ingredients of drugs with good efficacy in treating vomiting from pure traditional Chinese medicine preparations are rare at present.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective part of small Pinellia ternate decoction, preparation method thereof and application thereof
  • Effective part of small Pinellia ternate decoction, preparation method thereof and application thereof
  • Effective part of small Pinellia ternate decoction, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Mix pinellia and ginger evenly in a weight ratio of 1:1, add water (the weight of water is 5 to 8 times the total weight of pinellia and ginger) to extract for 3 to 5 hours, filter to obtain the extract, and continue to add water to the dregs (the weight of water 3 to 5 times the total weight of pinellia and ginger), extract for 2 to 3 hours, and combine the two extracts; add ethanol to the extract until the alcohol mass percentage is 60 to 80%, let it stand for 8 to 12 hours, centrifuge, and take Supernatant, concentrated to 1-2g crude drug·mL -1 , the concentrated solution was extracted twice with diethyl ether (each time the volume of diethyl ether was 0.8 to 1 times the volume of the concentrated solution), to obtain a diethyl ether layer and a water layer, and the diethyl ether layer was concentrated in a water bath under normal pressure at a concentration temperature of 39 to 49°C. A yellow oily substance is obtained, and the fat-soluble part is obtained.

[0029...

experiment example 2

[0030] Experimental Example 2: Determination of the content of chemical components in the active part of the present invention.

[0031] 1. Experimental materials:

[0032] 1.1 Sample: The active fraction obtained in Example 1 is the fat-soluble fraction, and three batches of the fat-soluble fraction (Lot 1, Lot 2, and Lot 3) were prepared.

[0033] 1.2 Instruments: Waters2690 high performance liquid chromatography, 996PDA detector, Millennium32 chromatography management system; Sartorius BP211D electronic balance.

[0034] 1.3 Reagents: Methanol is chromatographically pure, and water is sub-boiling distilled water (self-made).

[0035] 1.4 Reference substances: 6-gingerol and 6-shogaol reference substances are yellow oils obtained by separation and purification in our laboratory. The structure is determined by UV, IR, MS, NMR, etc., and it is detected as a single component by HPLC. The purity reaches more than 99%.

[0036] 2. Methods and results:

[0037] 2.1 Chromatogra...

experiment example 3

[0063] Experimental Example 3: The pharmacological effect of the effective part of the present invention on the treatment of vomiting.

[0064] 1. Effect on pigeon vomiting caused by copper sulfate and cisplatin.

[0065] 1. Experimental materials

[0066] 1.1 Sample, ① The active part obtained in Example 1 is the fat-soluble part, and it is prepared into a solution with a certain concentration before use. Preparation method: Take an appropriate amount of fat-soluble part, weigh it accurately, add a certain amount of 0.5% CMC-Na, ultrasonicate for 10 minutes, and stir until fully suspended; ②Metoclopramide; ③Ondansetron.

[0067] 1.2 Reagents, copper sulfate (analytical grade, Nanjing Chemical Reagent Factory); physiological saline (Nanjing Xiaoying Pharmaceutical Factory); cisplatin for injection (freeze-dried type) (produced by Shandong Jinan Qilu Pharmaceutical Co., Ltd., 20mg / bottle, batch number: 6040151 ), dissolved in normal saline to prepare 1.0mg·mL before use -1 s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an effective part of a small Pinellia ternate decoction. The effective part has the following physical and chemical properties: the effective part is a yellow oily substance, and the effective part which has certain volatility can be dissolved in hot water and ethanol; and the effective part which mainly contains gingerol and shogaol components concretely contains 40-50% of 6-gingerol and 12-20% of 6-shogaol. The invention also discloses a preparation method and an application of the effective part of the small Pinellia ternate decoction. The fat-soluble effective partextracted from the small Pinellia ternate decoction has the following obvious pharmacological drug effects: 1, the vomiting prevention effect is obvious, results of experiments of pigeon vomiting caused by copper sulfate and a chemotherapeutic cis-platinum as experiment models show that the fat-soluble part has a substantial vomiting prevention activity and can obviously reduce the vomiting frequency of pigeons; and 2, the effective part has a normal mouse stomach evacuation inhibition effect.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an effective part of Xiaobanxia decoction and its preparation method and application. Background technique [0002] Vomiting is a common clinical phenomenon, especially for cancer chemotherapy patients. Many patients often have to give up chemotherapy or radiotherapy due to fear of postoperative vomiting, thus delaying their illness. Although there are many drugs for treating vomiting at present, the effect is not ideal. Although drugs such as ondansetron and granisetron have certain effects on chemotherapy vomiting, these drugs are expensive and have certain side effects. It also limits their use to a certain extent. [0003] Vomiting belongs to the category of "qi inversion" in traditional Chinese medicine. It is believed that vomiting is caused by the loss of stomach harmony and the upward inversion of stomach qi. Traditional Chinese medicin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9068A61P1/08
Inventor 张科卫蔡皓徐春蕾陈文星刘晓
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products