Screening method of sewage treatment engineering bacterial strain and culture medium for screening

A technology for screening medium and sewage treatment, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of few types of microorganisms, loss of strains, etc.

Inactive Publication Date: 2010-03-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the strains of bacteria with excellent water treatment performance are lost due to the small number of microorganisms isolated by the current screening method of sewage treatment engineering bacteria strains, and provide a screening method and screening method for sewage treatment engineering bacteria strains. culture medium

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0004] Specific embodiment one: the medium used for the screening of sewage treatment engineering bacteria strains in this embodiment includes two kinds of liquid screening medium and solid screening medium; ~2gK 2 HPO 4 , 0.25~0.75gKH 2 PO 4 , 0.3~0.5gNH 4 NO 3 , 0.1~0.3gMgSO 4 ·7H 2 O. 100-995mL is made from untreated sewage sterilized at 112-130°C for 10-30min and remaining distilled water; each 1000mL solid screening medium consists of 0.4-0.6g peptone, 0.05-0.15g yeast, 1-2g K 2 HPO 4 , 0.25~0.75gKH 2 PO 4 , 0.3~0.5gNH 4 NO 3 , 0.1~0.3gMgSO 4 ·7H 2 O. 14-16g of agar, 100-980mL of untreated sewage sterilized at 112-130°C for 10-30 minutes and the rest of distilled water.

specific Embodiment approach 2

[0005] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the sewage treatment engineering bacteria strain screening medium includes two kinds of liquid screening medium and solid screening medium; every 1000mL liquid screening medium consists of 0.5g peptone , 0.1g yeast, 1.5g K 2 HPO 4 , 0.5gKH 2 PO 4 , 0.4gNH 4 NO 3 , 0.2gMgSO 4 ·7H 2 O. 120-970mL is made of sewage to be treated and the rest of distilled water sterilized at 115-128°C for 12-28min; each 1000mL solid screening medium consists of 0.5g peptone, 0.1g yeast, 1.5g K 2 HPO 4 , 0.5gKH 2 PO 4 , 0.4gNH 4 NO 3 , 0.2gMgSO 4 ·7H 2 O. 15g of agar, 120-960mL of untreated sewage sterilized at 115-128°C for 12-28 minutes and the rest of distilled water.

specific Embodiment approach 3

[0006] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that 150-950 mL of untreated sewage sterilized at 118-125° C. for 15-25 minutes is added to every 1000 mL of liquid screening medium. Others are the same as the first or second embodiment.

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Abstract

The invention relates the screening method for treating strain and culture medium. The invention solves the problems that the now screening method gets little kinds of microbios and strain is lost. The culture medium comprises liquid and solid culture mediums. The screening method comprises the following steps: 1 choosing activated sludge; 2 making culture medium; 3 enriching and domestication culture medium; 4 diluting mixture of culture medium and disinfection water, then applying them on the solid culture medium; 5 choosing plates to culture; 6 separating strain, purifying; 7 checking, andchoosing good strains. The strain species from the said method is 3-4 times than that from other method.

Description

technical field [0001] The invention relates to a screening method of bacterial strains and a medium for screening. Background technique [0002] Immobilized microorganism technology is widely used in the field of water treatment. The water treatment performance of engineering bacteria strains in immobilized microorganism technology has a great influence on the final sewage treatment effect. However, the current screening methods for sewage treatment engineering bacteria strains use LB medium to enrich and isolate bacteria, and the microorganisms that can grow and reproduce on LB medium only account for a small part of the total bacterial population, so many sewage treatment performances are excellent. Loss of strains leading to selection failure. Contents of the invention [0003] The purpose of the present invention is to solve the problem that the strains of bacteria with excellent water treatment performance are lost due to the small number of microorganisms isolated ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
Inventor 马放苏俊峰杨基先高珊珊
Owner HARBIN INST OF TECH
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