Amphioxus uncoupling protein gene and application thereof

Abstract

The invention relates to an amphioxus uncoupling protein (UCP) molecule clone and the functional research in the biotechnology field. The invention clones uncoupling protein (UCP) gene from amphioxus firstly with degeneracy primer and 3', 5'-RACE, which expresses muscle, intestinal canal and gill widely. Heightening UCP expression on the third day with dealing in low temperature indicates UCP engaging in body heat regulation in low temperature; Reducing UCP expression with hungry for fourteen days indicates that UCP engages in energy metabolism regulation. UCP is related to many diseases as diabetes, cardiovascular disease, tumour and so on, so UCP can be produced medicines treating these diseases.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to molecular cloning and functional research of amphioxus uncoupling protein (UCP). 2. Background technology [0002] The main function of mitochondria is to participate in energy metabolism. When mitochondria respire, the respiratory chain transfers electrons and pumps protons from the mitochondrial matrix to the membrane space, forming the mitochondrial transmembrane potential. Protons return to the mitochondrial matrix by driving ATP synthase to synthesize ATP, forming a coupling of oxidation and phosphorylation. UCP localized in the mitochondrial inner membrane can leak protons from the mitochondrial intermembrane space back into the matrix, thereby reducing the membrane potential of mitochondria and uncoupling oxidation and phosphorylation. A total of 5 UCP family members have been found so far. Since UCP4 and UCP5 are far from the homology of the other 3 members, ...

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