Hongkong sea-gull shape bacterium inspection technology

A seagull technology, applied in the field of inspection technology, can solve problems such as difficult detection, low detection rate, and complicated detection procedures, and achieve the effect of time-saving and rapid inspection

A seagull technology, applied in the field of inspection technology, can solve problems such as difficult detection, low detection rate, and complicated detection procedures, and achieve the effect of time-saving and rapid inspection

CN101063164AInactive Publication Date: 2007-10-31杭州市疾病预防控制中心 +1
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  • Hongkong sea-gull shape bacterium inspection technology

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[0018] Take the fresh feces of patient A with diarrhea with a sterile cotton swab, insert it into the test tube of Cary-Blair transport medium, and send it to the laboratory for streak culture. Stool samples from human were inoculated on MacConkey medium with a concentration of 16 mg / L cefoperazone, and then placed in an incubator at a temperature of 37°C. After 48 hours of incubation, the petri dish was taken out and suspicious colonies were looked for with the naked eye, Hong Kong Gull-shaped bacteria colonies on this medium are colorless, transparent, flat, tiny colonies with a diameter between 0.5mm and 1.0mm; after selecting these suspicious colonies, they are purified and cultured, and then the experiment is carried out according to the following steps: first Use the conventional Gram staining bacteria test procedure, and the test result is negative for short bacillus or vibrio, and then carry out the following tests; secondly, after the routine oxidase test and catalase ...

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Abstract

The invention discloses a testing technique of HongKong sea-gull shape bacteria, which comprises the following steps: fetching faecal specimen; seeding on MaiKangKai medium with cefoperazone; putting into 37 deg. c box for 48 h; taking-up; sorting colourless, transparent and flat dwarf colony with diameter at 0. 5-1. 0mm; proceeding purified culture; testing as negative bacillus brevis or vibrio with Gram's stain; checking as positive with oxidase and catalase; seeding in triose metal medium tube; placing in 37 deg. c box; culturing for 24 h; taking-up; keeping the colour of fresh red medium; or changing more red; diagnosing fitting result bacteria with PCR technique; diagnosing as HongKong sea-gull shape bacteria when PCR result as positive. This invention provides criterion for prevention and cure or clinical therapy of HongKong sea-gull shape bacteria disease, which is convenient, practical and rapid.

Description

technical field [0001] The invention relates to an inspection technology, in particular to a bacteria inspection technology. Background technique [0002] Gulliformis is a new enteropathogenic bacterium first discovered in Hong Kong in 2001. After in-depth research, it was found that this bacterium mainly causes diarrhea in infected persons, and eating freshwater fish and traveling are risk factors for this bacterial infection . Since this is a newly discovered bacterium, a set of standardized, effective, and fast inspection techniques and inspection procedures for this bacterium have not yet been established. In Hong Kong, more than ten biochemical tests are required for the inspection of Gull-shaped bacteria in Hong Kong, so the detection rate is low, it is difficult to find, and the detection procedure is too complicated. Contents of the invention [0003] The purpose of the present invention is to provide a simple, practical and rapid Hong Kong gull-shaped bacteria d...

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Application Information

Patent Timeline
31 Oct 2007
Publication
CN101063164A
IPC
C12Q1/04; C12Q1/26; C12Q1/68
Inventors
孙建荣; 项海青