Plate for biological sample analysis

Abstract

When a biological sample is moved by rotating a biological sample analysis plate, the plate can move the sample easily from the outer periphery side to the inner periphery side of the center of rotation of the plate. A chamber section (16) provided on the inner periphery side relative to the center of rotation of a plate and a sample holding section (20) provided on the outer periphery side of the plate are connected by flow paths (17, 18), air is previously held inside the sample holding plate (20), and, when a biological sample is moved from the chamber (16) toward the sample holding section (20) by centrifugal force generated by the rotation of the plate, the air inside the sample holding chamber (20) is compressed and held in the sample holding section (20).

Description

technical field [0001] The present invention relates to a plate for biological sample analysis that moves DNA, protein, and other biological samples in a buffer, detects their transport reaction, and discriminates biological samples. Background technique [0002] When it comes to general biological samples, there are roughly DNA and proteins. In addition, due to the rapid development of molecular biology in recent years, the degree of correlation of genes in various diseases has been quite accurately understood, and medical treatment targeting genes is attracting attention. Regarding DNA, the current focus is on SNPs (single nucleotide polymorphism, generally translated into "single nucleotide polymorphism", which is a general term for the differences in single codes (single bases) in genes). The reason is that, through the classification of SNPs, the prevalence of many diseases, the effect or sensitivity of drugs to each person can be predicted, and even parents, children,...

AI Technical Summary

Problems solved by technology

[0017] In addition, in the case of a device using such a capillary, it is structurally difficult to perform electrophoresis in a short flow path, so it takes a long time to perform measurement with an excessively long swimming distance.

[0018] Moreover, even in the case of using the flow path on the plate, in order to separate the sample, for example, by using the method described in Patent Document 5 and Patent Document 6, a plurality of capillary channels are intersected and at least three electrodes are provided, and a voltage is applied to the Two electrodes among the at least three electrodes provided pass through the intersection to move the sample. However, in this method, since the flow paths intersect, the sample may not be electrophoresed smoothly. There is a problem that correct measurement results cannot be obtained

[0019] In addition, for example, in the methods described in Patent Document 3 and Patent Document 4, a small channel is buried on a platform, and by changing the rotational speed of the platform, the centripetal force generated by the rotation is changed to move the sample. The method has the problem that the sample can only be moved in the centripetal direction, and also has the problem that the shape of the thin channel is quite complicated

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