Method for cultivating black rot resisting cabbage vegetable

Abstract

The invention discloses a culture method of anti-black-rot cabbage vegetable, which comprises the steps: 1) cells of an anti-blact-rot Brassica nigra and cells of cabbage vegetable are cell fused to obtain hybridized cells; 2) the hybridized cells are in vitro cultured, and regenerated plant is obtained, so the anti-black-rot cabbage vegetable is obtained. The method of the invention adopts cell hybridization technique, overcomes the interspecific hybridization obstacles in breeding, transplants the disease-fighting gene in cruciferae wild Brassica nigra into the gene cluster of cabbage vegetable, and thus culturing the disease-fighting cabbage vegetable such as anti-black-rot cauliflower.

Description

technical field [0001] The invention relates to a method for cultivating cabbage vegetables resistant to black rot. Background technique [0002] Cabbage vegetables include cabbage, cauliflower, broccoli, kohlrabi, kale, Brussels sprouts, kale, etc., which are the main vegetable groups in my country and occupy an important position in the annual supply of vegetables and foreign trade exports. Black rot is one of the main diseases that have long endangered the production of cabbage vegetables in my country. Its pathogenic bacteria have 6 major physiological races, of which physiological races 1 and 4 are the main pathogenic races, but the current cultivated species Ubiquitous is resistance to race 3 (Vicente, J.G., Conway, Roberts, S.J., and Taylor, J.D. Identification and origin of Xanthomonas campestris pv campestris races and related pathovars. Phytopathology, 2001, 91: 492-499; Vicente , J G., Taylor J D, Sharpe A G, Parkin I A P, Lydiate D J, King G J. Inheritance of race...

AI Technical Summary

Problems solved by technology

However, many disease resistances are controlled by multiple genes. Only one gene is isolated and introduced, and the disease resistance obtained by transgenic materials is often unsatisfactory.

Moreover, the analysis, positioning, cloning and transformation of resistance genes have problems such as high technical difficulty, long cycle, high investment, and market access and acceptance.

Another approach is to introduce resistance genes into cultivars through sexual crossing, but reproductive isolation between species makes routine sexual crossing very difficult or impossible

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