Application of double coptis in restraining apoptosis of virus induction MDCK cell

A technology of Shuanghuanglian and cells, which is applied in the application field of Shuanghuanglian preparations in inhibiting virus-induced MDCK cell apoptosis, and can solve problems affecting influenza virus host cells and other issues

Inactive Publication Date: 2008-02-27
孙坚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether Shuanghuanglian can affect the host cells of influenza virus an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Application of Shuanghuanglian preparation in inhibiting virus-induced apoptosis of MDCK cells.

Embodiment 2

[0050] Embodiment 2, MDCK cell culture fluid, wherein: the MDCK cell culture fluid is a digestive fluid composed of 0.22% volume concentration of trypsin and 0.03% volume percentage concentration of EDTA.

Embodiment 3

[0051] Embodiment 3, the detection method of MDCK cell apoptosis, is characterized in that operate according to the following steps:

[0052] A. Preparation of cells

[0053] ① Take a bottle of MDCK cells grown into a monolayer, discard the growth medium, and wash three times with phosphate buffered saline (PBS);

[0054] ②Add 0.22% trypsin by volume and 0.03% EDTA to incubate at 37°C for 5-10 minutes, shake gently until the cells fall off;

[0055] ③ Add 10% fetal calf serum to the cell growth medium of DMEM to make cell suspension;

[0056] ④ Dilute the MDCK cells to the desired concentration and distribute them in cell culture flasks, 24-well plates and 96-well cell culture plates for use;

[0057] B. Preparation of influenza virus

[0058] ① Take influenza A1 virus frozen at -70°C and add diluent to make a 10-fold serial dilution to obtain a diluted virus solution; the diluent is DMEM with a volume percentage concentration of 4% albumin;

[0059] ② Inoculate MDCK cells...

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Abstract

The invention discloses an application of dixanthogenate to inhibit virus to induce MDCK cell death and detecting method of MDCK cell death, which is characterized by the following: the MDCK cell culture liquid is digestive liquid of 0. 22% tryptone and 0. 03% EDTA; the MDCK cell can be target cell to test the influence of dixanthogenate for cell death through amphophilic method on the stream-oriented cell device; the cell death rate has obvious difference (P<0. 01), which proves the cell death induced by influenza virus inhibited by dixanthogenate; the new target is found firstly in the anti-influenza virus of dixanthogenate.

Description

technical field [0001] The invention relates to the application of Shuanghuanglian preparation in inhibiting virus-induced MDCK cell apoptosis and a detection method for MDCK cell apoptosis. Background technique [0002] The detection method of MDCK cell apoptosis is helpful for screening drugs for treating viral diseases. The application of Shuanghuanglian preparation in inhibiting virus-induced apoptosis of MDCK cells has not been reported yet. [0003] Influenza is called flu for short. Influenza virus is the pathogen of influenza. Influenza virus is very easy to mutate and form new virus strains. Because the population has no immunity to new strains, it often causes epidemics and is very harmful to humans. Therefore, research on anti-influenza virus drugs has attracted much attention. . Since the 1960s, anti-influenza virus drugs have appeared one after another. Commonly used are ① ion channel agents, whose target is influenza virus matrix protein-2, such as amantadin...

Claims

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Application Information

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IPC IPC(8): A61K36/634A61P31/16C12Q1/18
Inventor 孙坚何士勤王农荣杨斌
Owner 孙坚
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