On-off total saponin as well as its extracting method and application

An extraction method and technology for total saponins, which are applied in the field of extraction of discontinuous total saponins, can solve problems such as less research on pharmacological effects, and achieve the effects of facilitating sample quality control and evaluation and clear components.

Active Publication Date: 2008-04-16
GUANGZHOU BOJI MEDICINE SERVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than 20 saponins have been isolated from Dipsacus, among which 3-O-α-L-arabinopyranosylhederidenin-28-O-β-D-glucopyranose (1- 6)-β-D-Glucopyranoside (Asperosaponin VI) has the highest content, but there are few studies on the pharmacological effects of its various or individual components at home and abroad

Method used

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  • On-off total saponin as well as its extracting method and application
  • On-off total saponin as well as its extracting method and application
  • On-off total saponin as well as its extracting method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Take 1 kg of Dipsacus, extract twice with 8L 60% ethanol, reflux for 2 hours each time, combine the extracts, concentrate under reduced pressure, recover the ethanol until there is no alcohol smell, add water to dilute to a liquid containing 0.6g of medicinal materials per ml, and let it stand for 24 hour, take the supernatant, and use a hollow fiber ultrafiltration with a molecular weight of 10,000 to obtain a filtrate. Add the filtrate to the chromatographic column of 1.2kgD101 macroporous resin, and after cyclic adsorption for 3 times, use 4L 0.5% NaOH to elute, then wash with 5.5L water until nearly neutral, discard the first two eluents, and then use 6L Rinse with 60% ethanol, collect the 60% ethanol eluate, recover the ethanol under reduced pressure, concentrate and dry to obtain 102 grams of total saponins in Dipsacus, with a total saponin content of 85.7%, of which XDS-VI content is 60.8%.

Embodiment 2

[0038] Take 1 kg of Dipsacus, extract twice with 6L 80% ethanol, reflux for 2 hours each time, combine the extracts, concentrate under reduced pressure, recover the ethanol until there is no alcohol smell, add water to dilute to a liquid containing 1.0g of medicinal materials per ml, and let it stand for 36 hours, take the supernatant, and use Al 2 o 3 Ceramic membrane microfiltration to obtain the filtrate. Add the filtrate to the chromatographic column of 1.0kg AB-8 macroporous resin, after cyclic adsorption twice, elute with 3L of 0.5% NaOH, rinse with 5L of water, discard the eluent, and rinse with 5L of 80% ethanol , collect the eluate, recover ethanol under reduced pressure, concentrate and dry to obtain 98 grams of total saponins of Dipsacus cuspidatum, with a total saponin content of 78.3% and an XDS-VI content of 53.2%.

Embodiment 3

[0040] Take 1 kg of Dipsacus, extract twice with 6L 90% ethanol, reflux for 3 hours each time, combine the extracts, concentrate under reduced pressure, recover the ethanol until there is no alcohol smell, add water to dilute to a liquid containing 1.2g of medicinal materials per ml, and let it stand for 36 hour, take the supernatant, and filter it with a 0.45 μm filter membrane to obtain the filtrate. Add the filtrate to the chromatographic column of 1.2kg D201 macroporous resin. 3 Elution, then rinse with 6L of water, discard the eluate, then rinse with 4L of 95% ethanol, collect the eluate, recover ethanol under reduced pressure, concentrate and dry to obtain 95 grams of total saponins in Dipsacus, with a total saponin content of 85.8% , XDS-VI content 71.9%.

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Abstract

A dipsacus root total saponin is disclosed in the present invention, its weight percent accounts for 60 percent to 95 percent of dipsacus root medicinal materials extraction gross, and in dipsacus root total saponin 3-O-alpha-L-arabinopyranosyl-hederagenin-28-O-beta-D-glucopyranosyl(1-6)-beta-D-glucopyranoside accounts for 30 percent to 90 percent of dipsacus root medicinal materials extraction. A dipsacus root extracting method and its application in preparing medicine for treating cervical vertebra, arthritis, hyperosteogeny or osteoporosis are also disclosed in the present invention. By researching pharmacologic effect of the dipsacus root total saponin, finding that the dipsacus root total saponin prepared by the present invention has obvious anti-inflammation, pain-relieving, increasing carotid blood flow and decreasing blood viscosity, treating oestrogen deficiency type osteoporosis function, and has obvious prevention and cure for experimental cervical spondylosis, arthritis, hyperosteogeny or osteoporosis.

Description

technical field [0001] The present invention relates to the extract of Dipsacaceae medicinal material-total saponins of Dipsacus, and meanwhile, the invention also relates to an extraction method and application of the total saponins of Dipsacus. Background technique [0002] Dipsacus is the dry root of Dipsacus asperoides.Y.Cheng et T.M.Ai, a plant of Dipsacaceae. It was first recorded in "Shen Nong's Materia Medica", listed as the top grade, and it is recorded that it is "bitter in taste, slightly warm, mainly for typhoid fever, tonic for insufficiency, golden sores, carbuncles, broken bones, continuation of muscles and bones, difficult for women to breastfeed, long-term use to benefit strength. A Long bean, one is broken." "Bielu of Famous Doctors" contains "a bone-setter." Li Shizhen said, "Dusuan, genus-fold, and bone-setter are all named after merit." From its name, we can see that Dipsacan has been a bone injury since ancient times. The department needs medicine. Re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P29/00A61P19/00
Inventor 马仁强庞建新刘璇邹红
Owner GUANGZHOU BOJI MEDICINE SERVICES
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