Goat mTOR gene cDNA encoding zone 3í» terminal nucleotide sequence

A technology of nucleotide sequence and coding region, applied in the field of cDNA, can solve the problems of no goat mTOR gene cDNA nucleotide sequence, no mTOR research report, etc.
CN101245346AInactive Publication Date: 2008-08-20旭日干 +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
旭日干
Publication Date
2008-08-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a cDNA which encodes mTOR protein and is separated from a goat testicle cell and the corresponding amino acid sequence. The invention finds a conservation region by comparing the nucleotide sequences of the known human, rats and mice mTOR genes and then designs a pair of degenerate primers for amplifying the 3' end fragment of a cDNA coding area of a goat mTOR gene by RT-PCR, the primers are used for amplifying the specific fragment by the PT-PCR method, the nucleotide sequence of 612bp of the 3' end of the cDNA coding area of the goat mTOR gene and the corresponding amino acid sequence are obtained after sequencing. The obtained 612bp nucleotide sequence can be further used for full-length cDNA cloning of the goat mTOR gene and detecting the tissue expression specificity of the mTOR gene by the RT-PCR or the hybridization method, which can also be used for preparing an antibody for detecting the mTOR after recombinant expression.
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Description

technical field

[0001] The present invention relates to cDNA encoding mTOR protein isolated from goat testicular cells, more specifically relates to the 3' terminal nucleotide sequence of the cDNA coding region encoding mTOR protein and its corresponding amino acid sequence. Background technique

[0002] TOR (target of rapamycin) belongs to the PIKK superfamily, functions as a Ser / Thr kinase, and was first discovered in yeast. In mammalian cells, it is called mTOR (the mammalian target of rapamycin) or FRAP, RAFT, RAPT, and the gene was first isolated and cloned in 1994. Unlike yeast, there is only one TOR gene in mammalian cells, but the mTOR protein forms two complexes with different components, called mTORC1 and mTORC2. mTORC1 is sensitive to rapamycin (rapamycin), mediates the signal transduction process related to nutritional factors, regulates the growth and proliferation of cells, and its function and regulation mechanism are well understood; mTORC2 is sensitive to r...

Claims

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