Distant hybridization method for megalobrama amblycephala and yellow catfish

Abstract

The invention discloses a method for the distant hybridization of megalobrama amblycephala and xenocypris daviodi bleeker; in the method, diploid megalobrama amblycephala (or xenocypris daviodi bleeker) is taken as the maternal ripe fish, and diploid xenocypris daviodi bleeker (or megalobrama amblycephala) is taken as the paternal ripe fish; fertilized eggs are incubated through the artificial dry fertilization to the maternal ripe fish and the paternal ripe fish; the incubated fry is used to feed, to obtain the hybrid variety of the megalobrama amblycephala and the xenocypris daviodi bleeker. The method of the invention not only improves the fertilization rate and the incubation rate of distant hybridization, and the obtained novel types have the advantages of elegant shape, tender fish,strong stress resistance, etc.; therefore, the method is significant in the research on fish genetic breeding and the theory of biological evolution in future.

Description

The method of distant hybridization between the bream and the yellow-tailed bream technical field The invention relates to a method for hybridization of fish, in particular to a method for distant hybridization between Cyprinidae and Cyprinidae in Cyprinidae. Background technique Distant hybridization refers to the hybridization between individuals of species or more than one species. At present, distant hybridization technology has become an important method of fish genetic breeding and trait improvement. Distant hybridization can transfer a complete set of exogenous genomes into eggs, thereby changing the expression regulation and interaction mode of hybrid offspring genes, so that offspring may show hybrids in terms of viability, growth potential, stress resistance, yield, quality, etc. However, distant hybridization is also prone to the problem that hybrid offspring cannot survive or have a low survival rate. Megalobramaamblycephala (Megalobramaamblycephala) belongs ...

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Problems solved by technology

Distant hybridization can transfer the entire set of foreign genomes into eggs, thereby changing the expression regulation and interaction mode of hybrid offspring genes, so that the offspring may show hybrids in terms of viability, growth potential, stress resistance, yield, and quality. Advantages, but distant hybrids are also prone to the problem that hybrid offspring cannot survive or have a low survival rate

Megalobrama amblycephala is a diploid fish (2n=48) belonging to Cyprinidae and Cyprinidae, which has the advantages of height and fast growth. The diploid fish (2n=48) has the advantages of tender meat and high meat content. These two fish have great differences in biological characteristics such as classification and body shape, so the distant hybridization between the two fish is relatively large. Difficult, and so far there has been no report of successful distant hybridization between Cyprinidae and Cyprinidae

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