Distant hybridization method for megalobrama amblycephala and yellow catfish

A technology of yellow-tailed pomfret and distant hybridization, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of remote hybridization difficulties, hybrid offspring cannot survive survival rate, etc., and achieve the improvement of hatching rate and growth speed Fast, meaty results

Active Publication Date: 2009-01-14
湖南岳麓山水产育种科技有限公司
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Problems solved by technology

Distant hybridization can transfer the entire set of foreign genomes into eggs, thereby changing the expression regulation and interaction mode of hybrid offspring genes, so that the offspring may show hybrids in terms of viability, growth potential, stress resistance, yield, and quality. Advantages, but distant hybrids are also prone to the problem that hybrid offspring cannot survive or have a low survival rate
Megalobrama amblycephala (Megalobrama amblycephala) belongs to Cyprinidae, a diploid fish (2n=48) in the subfamily Cyprinidae. The diploid fish (2n=48) in the family has the advantages of tender meat and high meat content. These two fish have great differences in biological characteristics such as classification and body shape, so there is a distant hybrid between the two It is quite difficult, and so far there is no report on the successful distant hybridization between the subfamily of Cyprinidae and the subfamily of Cyprinidae.

Method used

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  • Distant hybridization method for megalobrama amblycephala and yellow catfish
  • Distant hybridization method for megalobrama amblycephala and yellow catfish
  • Distant hybridization method for megalobrama amblycephala and yellow catfish

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Experimental program
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Embodiment

[0017] During the breeding season, female bream and yellow-tailed pomfret with excellent traits and more than two years old were selected and injected with a mixture of chorionic gonadotropin (HCG) and lutein-releasing hormone analogue (LRH-A) to induce labor. The dosage of HCG was 600IU / kg, and the dosage of LRH-A was 20μg / kg, both of which were injected into the thorax at an angle of 45° at the depression-free scale at the base of the pectoral fin. Then choose male bream and yellow-tailed pomfret, and inject the same mixture of chorionic gonadotropin (HCG) and lutein-releasing hormone analog (LRH-A), but the injection dose is halved. The injections are divided into two injections, the first dose is 1 / 5 of the total dose, the second injection is the rest, and the interval between the two injections is 10 hours.

[0018] Put the injected parent fish into the same spawning pond, and hang a mesh in the pond to observe whether the parent fish is spawning. About 10 hours after th...

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Abstract

The invention discloses a method for the distant hybridization of megalobrama amblycephala and xenocypris daviodi bleeker; in the method, diploid megalobrama amblycephala (or xenocypris daviodi bleeker) is taken as the maternal ripe fish, and diploid xenocypris daviodi bleeker (or megalobrama amblycephala) is taken as the paternal ripe fish; fertilized eggs are incubated through the artificial dry fertilization to the maternal ripe fish and the paternal ripe fish; the incubated fry is used to feed, to obtain the hybrid variety of the megalobrama amblycephala and the xenocypris daviodi bleeker. The method of the invention not only improves the fertilization rate and the incubation rate of distant hybridization, and the obtained novel types have the advantages of elegant shape, tender fish, strong stress resistance, etc.; therefore, the method is significant in the research on fish genetic breeding and the theory of biological evolution in future.

Description

technical field [0001] The invention relates to a hybridization method of fish, in particular to a method for distant hybridization between the subfamily of the carps and the subfamily of the subfamily of the carps. Background technique [0002] Distant hybridization refers to the hybridization between individuals of an interspecies or more than one species. At present, distant hybridization technology has become an important method for fish genetic breeding and trait improvement. Distant hybridization can transfer the entire set of foreign genomes into eggs, thereby changing the expression regulation and interaction mode of hybrid offspring genes, so that the offspring may show hybrids in terms of viability, growth potential, stress resistance, yield, and quality. Advantages, but distant hybrids are also prone to the problem that hybrid offspring cannot survive or have a low survival rate. Megalobrama amblycephala (Megalobrama amblycephala) belongs to Cyprinidae, a diploi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K67/027
CPCY02A40/81
Inventor 刘少军刘筠罗凯坤胡婕覃钦博肖军肖俊
Owner 湖南岳麓山水产育种科技有限公司
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