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Natural hybridization seed-production method of triploid radix salvia miltiorrhizae using purple gene as label character

A hybrid seed production and triploid technology, applied in plant genetic improvement, botanical equipment and methods, angiosperms/flowering plants, etc., can solve the problems of lack of repatriation and improvement, urgent development, etc., to eliminate artificial mixing and save Labor and production cost reduction effect

Inactive Publication Date: 2011-07-20
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lot of research work to be done urgently on the discovery, innovation and directional cultivation of new varieties of Danshen germplasm resources in my country.
Up to now, there is no unified fine variety of cultivated salvia miltiorrhiza in the whole country.

Method used

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  • Natural hybridization seed-production method of triploid radix salvia miltiorrhizae using purple gene as label character
  • Natural hybridization seed-production method of triploid radix salvia miltiorrhizae using purple gene as label character
  • Natural hybridization seed-production method of triploid radix salvia miltiorrhizae using purple gene as label character

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Embodiment Construction

[0027] Further illustrate the implementation of the present invention with example below: Example 1.

[0028] In the experimental base of Salvia miltiorrhiza in Jixian County, Tianjin, planting and field management were carried out according to conventional methods.

[0029] 1) The local tetraploid Salvia miltiorrhiza is used as the female parent, and the wild diploid Salvia miltiorrhiza from the Zhongtiaoshan area of ​​Shanxi Province is used as the male parent, and the father and mother are planted in the field at a ratio of 1:1; 3500 plants per mu.

[0030] figure 1 It is a tetraploid salvia miltiorrhiza. figure 2 It is diploid Salvia miltiorrhiza. image 3 It is a field planting diagram of natural hybridization of triploid Salvia miltiorrhiza, 4x♀:2x♂1:1. The left row of the picture is a tetraploid white-flowered salvia miltiorrhiza, and the right row is a diploid purple-flowered salvia miltiorrhiza.

[0031] 2) Carry out field management according to general conventi...

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Abstract

The invention relates to a natural-hybrid seed production method for triploid salvia which takes the purple genes as marker traits; the method takes tetraploid white salvia as the female parent and diploid purple salvia as the male parent; in the field, the male parent and the female parent are planted according to a proportion of 1 to 1; pollination is done by wild hornet and domesticated bees; 2 / 3 of the seeds produced on the tetraploid white salvia female parent plant are triploid salvia, while the remaining 1 / 3 are tetraploid white salvia; the hybrids with purple flowers are triploid salvia, while the hybrids with white flowers are tetraploid salvia; the triploid salvia is slightly purple at stems, leaves and petioles at seedling stage; the invention does not need manual castration, thereby reducing the labor intensity in seed production, saving the labor and decreasing the production cost; besides, the parental seeds can be accurately separated from the hybrid seeds, and the produced seeds have high purity. The invention can be applied to larger-area hybrid seed production and is easy to promote.

Description

technical field [0001] The invention relates to a hybrid seed production technology of triploid salvia miltiorrhiza, in particular to a method for producing natural hybrid seeds of triploid salvia miltiorrhiza with purple gene as a symbolic trait, specifically a natural hybrid seed production method with purple gene as a symbolic trait. Background technique [0002] Triploids are the only species that have two major advantages that exist in nature, namely polyploidy and heterosis. Triploid is the polyploid with the lowest ploidy among polyploids, but it has all the attributes of polyploid. Its origin is produced by 2n×n or n×2n hybridization, so it also has heterosis produced by hybridization. Therefore, in recent years, people have paid much attention to triploids and their advantages. Human beings have been using triploids for a long time, such as bananas, tulips with large flowers, and daffodils, which are all naturally occurring triploids. They have a history of hundre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00
Inventor 陈力李秀兰
Owner NANKAI UNIV
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