Technology for screening estrogen active compound by competitiveness enzyme-linked receptor compatible reaction

Abstract

The invention belongs to the technical field of assay and test and relates to a technology for rapid screening of estrogenic active compounds. The basic principle is to arrange test compounds, immobilized 17beta estradiol and estrogen receptor proteins ERalpha-LBD or ERbeta-LBD in the same reaction system to lead the test compounds and the immobilized 17beta estradiol to be competitively bound with the ERalpha-LBD or the ERbeta-LBD, and the number of the estrogen receptor proteins which are combined on the immobilized 17beta estradiol is finally detected by the enzyme-linked reaction. The basic evaluation basis of the estrogenic activity is to contrast the competitive binding capacities of the test compounds and the 17beta estradiol to the estrogen receptor proteins ERalpha-LBD or the ERbeta-LBD, thereby evaluating the estrogenic activity of the test compounds. The technology has wide application prospects in the aspects of toxicological studies of environmental pollutants, studies of metabolites of compounds, toxicity evaluation of industrial products, drug screening, analysis of active ingredients, etc.

Description

technical field [0001] The patent of the present invention belongs to the technical field of analysis and testing, and relates to a technology for rapid screening of estrogen-active compounds. This technology has broad application prospects in the toxicological research of environmental pollutants, the research of compound metabolites, the toxicity evaluation of industrial products, drug screening and active ingredient analysis. Background technique [0002] The health effects of environmental pollutants are a common concern. In recent years, more and more compounds that were considered "safe" and widely used have been proved to have estrogen or anti-estrogen activity, which can cause a series of estrogen-dependent physiological and biochemical process disorders, leading to experiments or wild animals. Abnormal embryonic development, decreased reproductive capacity, and abnormal sex differentiation. Although the pathways and biological effects of these compounds on organis...

AI Technical Summary

Problems solved by technology

Although this method has high accuracy, it is difficult for general laboratories to carry out due to the expensive reagents and the need for radioactive manipulation.

At present, there is no report on the technology of screening estrogen active compounds by competitive enzyme-linked receptor affinity reaction similar to the present invention