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Method for extracting broussonetia papyrifera leaf flavonoids

An extraction method and technology of leaf flavonoids are applied in pharmaceutical formulations, medical preparations containing active ingredients, and plant/algae/fungus/moss ingredients, etc., and can solve problems such as information on unseen products on the market.

Inactive Publication Date: 2009-04-29
HAINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on an effective extraction method and no information on the listing of such products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Mix 100g of pre-dried and pulverized leaves with 80% ethanol solution at a solid-to-liquid ratio of 1:20, extract at a leaching temperature of 90°C for 4 hours, extract 3 times with 30ml of chloroform, and remove impurities by static layering to obtain The aqueous phases were combined and then extracted 2-3 times with ethyl acetate, 25ml each time. The ethyl acetate extracts obtained after extraction were combined, and the ethyl acetate was evaporated by a rotary evaporator to obtain flavonoids. The yield of flavonoids was 6.93 mg.

Embodiment 2

[0016] Mix 100g of pre-dried and pulverized leaves with 70% ethanol solution at a solid-to-liquid ratio of 1:20, extract at a leaching temperature of 90°C for 4 hours, extract 3 times with 30ml of chloroform, and remove impurities by static layering to obtain The aqueous phases were combined and then extracted 2-3 times with ethyl acetate, 25ml each time. The ethyl acetate extracts obtained after extraction are combined, and the ethyl acetate is evaporated by a rotary evaporator to obtain flavonoids. The yield of flavonoids was 11.32 mg.

Embodiment 3

[0018] Mix 100g of pre-dried and pulverized leaves with 70% ethanol solution at a solid-to-liquid ratio of 1:30, extract at a temperature of 90°C for 3 hours, extract with chloroform three times, 30ml each time, and remove impurities by static layering to obtain The aqueous phases are combined and then extracted with ethyl acetate for 2 to 3 times, 25ml each time, the ethyl acetate extracts obtained after extraction are combined, and the ethyl acetate is evaporated by a rotary evaporator to obtain flavonoids. The yield of flavonoids was 38mg.

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Abstract

The invention relates to a method for extracting broussonetia papyrifera leaf flavonoid, which comprises the following steps: (a) cleaning, drying and crushing broussonetia papyrifera leaves, soaking the broussonetia papyrifera leaves with 60 to 95 percent ethanol solution according to the solid-liquid ratio of between 1 to 15 and 1 to 40 at room temperature for 12 to 48 hours; (b) heating and refluxing the materials in the step (a) for 3 to 6 hours to obtain a leaching liquor; (c) distilling off the ethanol in the leaching liquor prepared in the step (b) at a temperature of between 50 and 90 DEG C to obtain a concentrated solution; and (d) extracting the obtained concentrated solution by using chloroform for 3 times to obtain an aqueous phase which is extracted by ethyl acetate for 2 to 3 times, and distilling off the ethyl acetate from the obtained ethyl acetate extract after the extraction through a rotary evaporator, and the ethyl acetate is the flavonoid compound. The method is effective, simple and safe in the extraction and the separation of a natural product of the flavonoid compound, and is easy to realize industrial production.

Description

technical field [0001] The invention relates to the technology of extracting active ingredients from plants, in particular to the technical field of extracting flavonoids from the leaves of mulberry leaves. Background technique [0002] The mulberry tree, also known as the bark tree, hemp leaf tree, and vinegar peach tree, is a deciduous tree with strong adaptability, resistance to dry and cold, heat and humidity, developed root system, and few pests and diseases. It is widely distributed in our country and has natural resources. Comparative advantages, and the cultivation area is still expanding. The mulberry leaves contain high nutritional components, and the balance of various nutritional components is good, and its utilization value is high. [0003] The main components in the extract of the leaves of Structus truncifera are flavonoids. Flavonoids have good functions and effects of lowering blood sugar, regulating blood lipids, resisting lipid peroxidation, increasing ...

Claims

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Application Information

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IPC IPC(8): C07D311/40C07H17/07A61K36/60
Inventor 张玉苍何连芳孙岩峰孟岩
Owner HAINAN UNIVERSITY
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