Method for endothelial cell extraction from adipose tissues

Abstract

Adipose tissue has proven to serve as an abundant, accessible, and rich source of endothelial or vascular endothelial cells suitable for tissue engineering. We describe a detailed method for the isolation and purification of endothelial cells using purified enzymes and antibody-based selection. The cells can be obtained from liposuction procedures and used in vascular grafts.

Description

technical field [0001] The present invention relates to the field of cell culture and purification. In particular, the invention relates to endothelial cell culture and purification. Background technique [0002] For many applications in cell therapy and tissue engineering, endothelial cells need to be obtained from patient tissue for transplantation back into the same patient. Rapid isolation of large numbers of endothelial cells within a few hours is advantageous in order to minimize operating costs and to minimize any changes that may occur to endothelial cells during culture. In order for such endothelial cells to be practical, it is generally necessary to obtain large numbers of cells (eg, greater than 1 million) and to obtain isolated cells of relatively high purity (eg, greater than 90% endothelial identity). Furthermore, it is desirable to minimize separation time, which improves cell viability and increases ease of application. [0003] Bypass surgery is a common...

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However, EC recovery with CD34 beads was only about 24% (19)

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