Liver cancer patient postoperative transferring recrudescence polymolecular forecasting reagent kit based on inflammation factor

An inflammatory factor, multi-molecular technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of low prediction accuracy and unpredictable postoperative recurrence of early liver cancer.

Active Publication Date: 2009-05-27
SHANGHAI DIDA BIOTECH
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  • Application Information

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Problems solved by technology

At present, there are no sufficiently sensitive and specific predictors to predict the recurrence and survival of patients with liver cancer after surgery. Although alpha-fetoprotein, tumor size, and presence or absence of capsules are related to metastasis and recurrence, their prediction accuracy is overall. It is still very low, especially for the postoperative recurrence of early liver cancer, it is even more unpredictable

Method used

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  • Liver cancer patient postoperative transferring recrudescence polymolecular forecasting reagent kit based on inflammation factor
  • Liver cancer patient postoperative transferring recrudescence polymolecular forecasting reagent kit based on inflammation factor
  • Liver cancer patient postoperative transferring recrudescence polymolecular forecasting reagent kit based on inflammation factor

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Effect test

Embodiment 1

[0025] A kit for predicting metastasis and recurrence of liver cancer in patients with primary liver cancer after surgery, which consists of coating reagent, washing solution, horseradish peroxidase-labeled antibody, substrate solution, stop solution and prediction reference value;

[0026] Wherein the coating reagent is composed of pH9.6 carbonate coating buffer and interleukin 2 antibody, interleukin 15 antibody or interleukin 5 antibody;

[0027] Washing solution is pH7.4 phosphate buffer;

[0028] Horseradish peroxidase-conjugated antibodies consist of horseradish peroxidase-conjugated interleukin-2 antibody, horseradish peroxidase-conjugated interleukin-15 antibody and horseradish peroxidase-conjugated interleukin-5 Antibody composition;

[0029] The substrate solution is tetramethylbenzidine substrate solution;

[0030] The stop solution is 1.8M sulfuric acid;

[0031] The prediction reference value is based on the protein concentration of liver cancer pericancer tiss...

Embodiment 2

[0043] A kit for predicting metastasis and recurrence of liver cancer in patients with primary liver cancer after surgery, which consists of coating reagent, washing solution, horseradish peroxidase-labeled antibody, substrate solution, stop solution and prediction reference value;

[0044] Wherein the coating reagent is composed of pH9.6 carbonate coating buffer and interleukin 2 antibody, interleukin 15 antibody or interleukin 5 antibody;

[0045] The washing solution is pH7.4 phosphate buffer;

[0046] Horseradish peroxidase-conjugated antibodies consist of horseradish peroxidase-conjugated interleukin-2 antibody, horseradish peroxidase-conjugated interleukin-15 antibody and horseradish peroxidase-conjugated interleukin-5 Antibody composition;

[0047] The substrate solution is an ABTS substrate solution;

[0048] The stop solution is 2.1M sulfuric acid;

[0049] The prediction reference value is based on the protein concentration of liver cancer pericancerous tissue ext...

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Abstract

The invention provides a multimolecular prediction kit for postoperative recurrence and transfer for the patients with liver cancer based on inflammatory factor, characterized by comprising a coating agent, a washing liquid, a horseradish peroxidase-labeled antibody, a substrate solution, a stop solution, and prediction reference values, wherein, the coating agent is composed of a carbonate coating buffer solution of PH9.6, and an interleukin 2 antibody or an interleukin 15 antibody or an interleukin 5 antibody; based on the 4mug/mul of protein concentration of extract from liver peri-cancer tissue, the prediction reference values of interleukin 2, interleukin 15, and interleukin 5 are respectively 20-30pg/ml, 32-43pg/ml, and 18-28pg/ml, and the prediction reference values vary proportionably along with the sample concentration. In the invention, by using the interleukin 2, interleukin 15, and interleukin 5 in liver peri-cancer tissue, the postoperative recurrence and transfer for the patients with liver cancer can be accurately predicted.

Description

technical field [0001] The invention relates to a multi-molecule prediction kit for postoperative metastasis and recurrence of liver cancer patients based on inflammatory factors, which belongs to the field of biotechnology. Background technique [0002] Liver cancer is the second leading cause of cancer death in my country, and surgery is currently the main treatment. However, the high postoperative recurrence rate of metastasis (5-year recurrence rate reaches 70%, small liver cancer also reaches 50%) has become a bottleneck for further improving long-term efficacy, and is also an important key to conquer liver cancer. If it is possible to predict the early metastasis and recurrence of specific groups after radical resection of liver cancer, and to provide timely intervention and treatment, so as to achieve early detection and early treatment, the survival rate of patients with recurrence after radical resection will be further improved, and there will also be great social ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 周海军钦伦秀黄华
Owner SHANGHAI DIDA BIOTECH
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