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Mycoplasma pneumoniae rapid identification and cultivation susceptibility test kit and test method

A Mycoplasma pneumoniae and kit technology, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problems of time-consuming and labor-intensive, and achieve convenient use, ensure accuracy, and ensure stability Effect

Inactive Publication Date: 2009-10-14
钱叶林 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a rapid identification and culture drug sensitivity kit for Mycoplasma pneumoniae, which solves the time-consuming and labor-intensive problems existing in the existing diagnostic methods

Method used

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  • Mycoplasma pneumoniae rapid identification and cultivation susceptibility test kit and test method
  • Mycoplasma pneumoniae rapid identification and cultivation susceptibility test kit and test method
  • Mycoplasma pneumoniae rapid identification and cultivation susceptibility test kit and test method

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Embodiment

[0043]Take a piece of ultrapure water reagent 2 and a piece of Mycoplasma pneumoniae rapid identification medium 5, add 1.5ml of ultrapure water to Mycoplasma pneumoniae rapid identification medium 5, after completely dissolving, obtain a culture medium; take the culture medium obtained in the previous step Add 50 μl of solution to the negative detection hole 6 on the drug sensitivity plate; take a piece of saline reagent 4, use a cotton swab to absorb the saline, twist it several times at the lesion, take out the cotton swab, and immediately place it in step 1 In the obtained culture solution, stir the cotton swab several times, extract the cotton swab, squeeze out the liquid as much as possible against the bottle wall, take out the cotton swab, blow it several times with a sampler or shake it with an oscillator to mix well, and get Mixed culture solution; take 50 μl of the mixed culture solution obtained in the previous step, and add it to the positive detection well 7 and dr...

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Abstract

The invention discloses a mycoplasma pneumoniae rapid identification and cultivation susceptibility test kit which comprises a kit and a susceptibility panel, wherein, the kit comprises a box body which is internally provided with ultrapure water reagent, paraffin oil reagent, physiological saline reagent and mycoplasma pneumoniae rapid identification culture medium; the susceptibility panel includes a panel body and a panel cover, wherein, the panel body is provided with a negative test hole and a positive test hole, a plurality of drug property comparison holes are arranged correspondingly to the negative test hole and the positive test hole, and the drug property comparison holes corresponding to the positive test hole is internally provided with test medicine. The invention also discloses a test method which comprises: culture fluid is firstly prepared and then added into the negative test hole to extract pathological change solution, and the pathological change solution is added into the culture fluid to obtain mixed solution; the mixed solution is added into the positive test hole and the drug property comparison holes to be cultured, and then the result is obtained. The mycoplasma pneumoniae rapid identification and cultivation susceptibility test kit and the test method are simple in operation and short in detection period.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and specifically relates to a rapid identification and culture drug sensitivity kit for mycoplasma pneumoniae, and also relates to a detection method using the kit. Background technique [0002] Mycoplasma pneumoniae (M.Pneumonia) is a special microorganism, which is the pathogen of human mycoplasma pneumonia. Mycoplasma pneumoniae is neither a virus nor a bacterium, but a microorganism between viruses and bacteria. The pathological changes of mycoplasma pneumonia are mainly interstitial pneumonia, sometimes complicated by bronchial pneumonia, which is called primary atypical pneumonia. It is mainly transmitted by droplets, with an incubation period of 2 to 3 weeks, and the highest incidence rate is among adolescents. The clinical symptoms are mild, or even asymptomatic. If there are, there are only general respiratory symptoms such as headache, sore throat, fever, and cough, but the...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/35
Inventor 钱叶林王敏
Owner 钱叶林
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