Finger-print building method of radix cyathulae medicinal material and standard finger-print thereof

Abstract

The invention discloses a finger-print building method of radix cyathulae medicinal material, in which cyasterone serves as a reference substance, HPLC detection and gradient elution are carried out on reference substance solution and sample solution for tests under the same chromatographic condition, chromatogram map is recorded, thus obtaining the finger-print. The standard finger-print of radix cyathulae medicinal material obtained by the method of the invention improves the quality assessment system of radix cyathulae medicinal material and provides theoretic and practical basis for comprehensive effective quality control of the radix cyathulae medicinal material and decoction pieces thereof. The invention further discloses a standard finger-print of the radix cyathulae medicinal material obtained according to the above finger-print building method.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for constructing the fingerprints of Achyranthes bidentata medicinal materials and a standard fingerprint thereof. Background technique [0002] Although traditional Chinese medicine has a history of thousands of years of application and development, how to effectively evaluate the quality of traditional Chinese medicine / Chinese medicinal materials has always been a key and difficult issue in the research and application of traditional Chinese medicine. With the development of science and technology, fingerprint quality control technology has been proposed and widely recognized in the field of traditional Chinese medicine, and its application in quality control of Chinese medicinal materials and their extracts / preparations is also increasing. Product quality control and evaluation provide new ideas and methods. [0003] Ju...

AI Technical Summary

Problems solved by technology

However, the infrared spectrum is caused by the vibration-rotation transition of molecules, and it is difficult to determine its specificity and characteristics.

[0010] (6) Ultraviolet spectroscopy: It is an electronic spectrum with little change in shape, sometimes different compounds have similar spectra, and the specificity is poor

[0015] Due to the mixed varieties in the cultivation process, the confusion of varieties caused by the different medication habits in different regions, and the harvest / transportation / storage conditions, etc., the commercial varieties of Achyranthes bidentata are relatively chaotic, and their quality is not uniform, sometimes very different.

On the other hand, under the item of "Chuan Achyranthes bidentata" recorded in the 2005 edition of the Pharmacopoeia of the People's Republic of China, the quality control of Achyranthes bidentata is also very weak, including only microscopic identification and conventional methods such as moisture, ash, and extracts. There is no comprehensive quality evaluation system, and it is difficult to effectively control the quality of Chuan Achyranthes bidentata

Images