Endophyte with insecticidal active substances and application in biological control

Abstract

The invention discloses endophyte Claviceps purpurea CGMCC.No3073 and Pseudomonas fluorescens CGMCC.No3026 with insecticidal active substances and an application in biological control. The Claviceps purpurea obtains the optimal insecticidal pH of 7 to 8 by separating, screening and culturing in an achnatherum inebrians root, can maintain higher insecticidal activity in the pH range of 7.2-7.4 andhas the insecticidal activity in the temperature range of 20 DEG C-37 DEG C; and the Pseudomonas fluorescens has the optimal insecticidal pH of 7 to 8, can maintain higher insecticidal activity in the pH range of 7.2-7.4 and has the insecticidal activity in the temperature range of 20 DEG C-37 DEG C. A bacterial strain of the invention can be widely applied to the biological control field of controlling the damage of cotton bollworms, red spiders, locusts, and the like.

Description

Endophytic Bacteria with Insecticidal Active Substances and Its Application in Biocontrol field of invention The invention relates to the field of biological control, in particular, the invention relates to an endophytic bacterium with insecticidal effect on insect pests such as cotton bollworm, spider mite and locust and application thereof. Background technique More than 100 years ago, people have discovered that there are microorganisms in the interior of healthy plant tissues, and these microorganisms were later called plant endophytes (Endophytes) in the literature. At present, the more commonly used broad concept of endophytes was proposed by StoneJK et al., which refers to fungi or bacteria that live in various tissues and organs of healthy plants during a certain stage or all stages of their life history. The host plant does not (at least temporarily) exhibit extrinsic disease, and endophysis can be noted by histological methods or by isolation of microbial DNA fro...

AI Technical Summary

Problems solved by technology

At present, there is no report on the systematic research on the endophytic bacteria of Drunken Horse Grass. Due to the advantages of easy cultivation, easy control, low production cost, and fast growth, the microorganisms have been developed through methods such as mutagenesis and breeding to achieve large-scale industrialization. Grass endophytes become possible, and their application prospects are very broad

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