IF2 translation initiation factor gene relevant to wheat yellow rust resistance

A technology of translation initiation factor and stripe rust, which is applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problem of weak molecular basis research on wheat resistance to stripe rust, and achieve the effect of improving disease resistance

Inactive Publication Date: 2010-02-10
NORTHWEST A & F UNIV
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Problems solved by technology

[0004] However, the research on the molecular basis of wheat resistance to stripe rust is still relatively weak, and only Yr10 and Yr36 have been cloned so far (NCBI; Fu et al., 2009, Science)

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  • IF2 translation initiation factor gene relevant to wheat yellow rust resistance
  • IF2 translation initiation factor gene relevant to wheat yellow rust resistance
  • IF2 translation initiation factor gene relevant to wheat yellow rust resistance

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Embodiment Construction

[0018] In the present invention, ESTs are screened for genes related to stripe rust-resistant high-quality germplasm Shanmai 139 under biological stress conditions, so as to obtain genes related to the disease process of stripe rust resistance. Because when subjected to biological stress, the expression of genes related to resistance to stripe rust will be quite different from that before the stress, which is mainly reflected in the fact that crops respond to changes in the external environment and produce a series of physiological and biochemical reactions to adapt to changes in living conditions. Any change in an organism is done on the basis of gene expression. Therefore, the suppression subtractive hybridization (SSH) technique is used to reduce the background of gene expression in the unstressed state, so that genes involved in the disease process after stress can be screened and cloned. The selected Shaanxi wheat 139 (Ren Zhilong, Ji Wanquan, Zhang Hong, 2007 No. 5, Chin...

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Abstract

The invention discloses an IF2 translation initiation factor gene relevant to wheat yellow rust resistance. The overall length of a cDNA sequence of the gene is 1810bp, and a nucleotide sequence of the gene is shown as SEQ.ID.NO.1, wherein 1-165bp is a 5' non-translated region; 166-1386bp codes 406 kinds of amino acid, 1513-1665bp codes 50 kinds of amino acid, and 1666-1810bp is a 3' non-translated region. The gene is a conservative gene of wheat yellow rust resistance; RT-PCR expression analysis indicates that the gene has trace expressions under the normal condition, the induction expressionis increased after duress for 24 hours in a CY32 bacterial system, and the expression reaches the maximum for 72 hours and then is reduced in the following 4-7 days; disease resistant wheat leaves can display a symptom of suffering from puccinia striiformis infection by silencing the expression of the gene in yellow rust resistant wheat by an RNA interference technology.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to a wheat stripe rust resistance IF2 translation initiation factor gene. Background technique [0002] Wheat stripe rust is one of the main diseases of wheat production, which seriously affects the high and stable yield of wheat. Although timely chemical control can effectively control the damage of stripe rust on the basis of forecasting, the environmental pollution and ecological damage caused by chemical control have caused widespread focus on. Years of practice have proved that the use of rust-resistant wheat varieties is the most economical and effective way to control wheat stripe rust and increase wheat yield. However, wheat stripe rust strains mutate rapidly, and resistant varieties often lose resistance due to the adaptation of the pathogen. Therefore, obtaining reliable and long-lasting resistance to wheat stripe rust is the core of wheat breeding and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29
Inventor 吉万全张宏王长有王亚娟张荣琦陈春环朱建峰杨群慧
Owner NORTHWEST A & F UNIV
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