Acetyl coenzyme A synthetase gene relevant to wheat yellow rust resistance

Abstract

The invention discloses an acetyl coenzyme A synthetase gene similar to a wheat yellow rust resistant relevant gene. An overall length cDNA sequence of the gene is 1619bp, wherein a 3' non-translatedregion is 187bp, a 5' non-translated region is 136bp, a coding region is 1269bp, and 431 kings of amino acid are coded; the RT-PCR expression analysis of the gene indicates that the gene has trace expressions under the normal condition, the induction expression is increased after duress for 24 hours in a CY32 bacterial system, and the expression reaches the maximum for 72 hours, is sharply reducedin the following 4th day and rises in the 7th-13th day. The gene is an upstream gene of genes, such as S17, S120, S176, and the like, has the negative regulate and control effect on hypothetical protein S108 and induces the expression compensation of an S239 family gene. The gene transforms crops of wheat, corn, and the like and other plans, improves the yellow rust resistant reaction ability ofthe crops and has great application potential.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to an acetyl-CoA synthetase gene related to wheat stripe rust resistance. Background technique [0002] Wheat stripe rust is one of the main diseases of wheat production, which seriously affects the high and stable yield of wheat. Although timely chemical control can effectively control the damage of stripe rust on the basis of forecasting, the environmental pollution and ecological damage caused by chemical control have caused widespread focus on. Years of practice have proved that the use of rust-resistant wheat varieties is the most economical and effective way to control wheat stripe rust and increase wheat yield. However, wheat stripe rust strains mutate rapidly, and resistant varieties often lose resistance due to the adaptation of the pathogen. Therefore, obtaining reliable and long-lasting resistance to wheat stripe rust is the core of wheat breeding and p...

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Problems solved by technology

[0004] However, the research on the molecular basis of wheat resistance to stripe rust is still relatively weak, and only Yr10 and Yr36 have been cloned so far (NCBI; Fu et al., 2009, Science)

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