Recombined adenovirus expressing Asia-1 foot-and-mouth disease virus VLP (Virus-like Particles) and application thereof

Abstract

The invention discloses a recombined adenovirus expressing Asia-1 foot-and-mouth disease virus VLP (Virus-like Particles) and application thereof. The recombined adenovirus is obtained by the following steps of: splicing an FMDV (Foot-and-Mouth Disease Virus) capsid protein precursor P1-2A gene and a 3C gene together to obtain a fusion gene, wherein a base sequence of the fusion gene is SEQ ID NO as shown in a table 1; connecting the base sequence of SEQ ID NO as shown in the table 1 with an adenovirus shuttle vector to obtain a recombined adenovirus shuttle vector; and recombining the recombined adenovirus shuttle vector into a human defective adenovirus-5, screening, and identifying to obtain the recombined adenovirus efficiently expressing the Asia-1 foot-and-mouth disease virus VLP, wherein the microbial preservation number of the recombined adenovirus is CGMCC No. 3467. The Asia-1 foot-and-mouth disease virus VLP established by the invention and expressed by a defective adenovirus can continuously induce high-level neutralizing antibodies in mice and can be used as vaccines for preventing Asia-1 foot-and-mouth diseases.

Description

technical field [0001] The present invention relates to a recombinant adenovirus, in particular to a recombinant adenovirus expressing Asian type 1 foot-and-mouth disease virus VLP. The present invention also relates to the construction method of the recombinant adenovirus and the application of the recombinant adenovirus in the prevention of foot-and-mouth disease, belonging to foot-and-mouth disease the field of prevention. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious infectious disease of cloven-hoofed animals, and its pathogen is foot-and-mouth disease virus (FMDV). In view of the serious harm caused by foot-and-mouth disease to the world economy, the International Office of Epizootics (OIE) and the United Nations Food and Agriculture Organization (FAO) listed it as the first class A infectious disease, and my country also listed it as the first class of animal infectious diseases. Vaccination is an effective means t...

AI Technical Summary

Problems solved by technology

Because FMDV is a highly mutated virus, the nucleotide sequences of different serotypes such as O-type and Asia1-type foot-and-mouth disease differ by more than 15%. In addition, the genome structure of FMDV is unique, and it is very difficult to clone and assemble correctly. It is technically difficult to express FMD virus-like particles and induce high levels of neutralizing antibodies in animals

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