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Quality control method for mulberry chrysanthemum granules

A detection method, the technology of Xia Sangju, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of single composition, ambiguity of quality control of Xia Sangju granules, etc., and achieve the effect of ensuring effectiveness

Active Publication Date: 2012-01-04
GUANGZHOU XINGQUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality control methods for Xiasangju granules in the prior art are not aimed at its monarch drug ingredient Prunella vulgaris, and the established fingerprints identify a single component, and the quality control of Xiasangju granules is ambiguous. Therefore, it is necessary to establish a perfect quality control method standard system

Method used

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  • Quality control method for mulberry chrysanthemum granules
  • Quality control method for mulberry chrysanthemum granules
  • Quality control method for mulberry chrysanthemum granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Adopt high performance liquid chromatography to measure the content of rosmarinic acid, comprise the following steps:

[0070] 1. Preparation of reference solution

[0071] Accurately weigh an appropriate amount of rosmarinic acid reference substance and dissolve it in methanol to obtain a reference substance solution with a concentration of 0.22 μg / μL.

[0072] 2. Preparation of the test solution

[0073] Weigh about 2.5g of Xiasangju granules, weigh them accurately, add 10mL of methanol, weigh, ultrasonicate for 30min, take it out, let it stand, let it cool, make up the weight, filter through a microporous membrane, and use it as the test sample.

[0074] 3. Determination by liquid chromatography

[0075] Accurately draw the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for determination.

[0076] Chromatographic conditions

[0077] Chromatographic column: Agilent Eclipse XDB-C18 (4.6mm×250mm, 5μm)...

Embodiment 2

[0100] Adopt high performance liquid chromatography to measure the content of rosmarinic acid, comprise the following steps:

[0101] 1. Preparation of reference substance solution: same as in Example 1.

[0102] 2. Preparation of the test solution: Weigh about 2.5g of Xiasangju granules, accurately weigh, add 10mL of methanol, weigh, ultrasonicate for 60 minutes, take it out, let it stand, let cool, make up the weight, filter through a microporous membrane, and use it as the test solution Taste.

[0103] 3. Determination by liquid chromatography

[0104] Accurately draw the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for determination.

[0105] Chromatographic conditions:

[0106] Chromatographic column: Agilent Eclipse XDB-C18 (4.6mm×250mm, 5μm);

[0107] Flow rate: 1.5mL.min -1 ;

[0108] Detection wavelength: 335nm;

[0109] Column temperature: 25°C;

[0110] Mobile phase: mobile phase A is aceton...

Embodiment 3

[0113] Adopt high performance liquid chromatography to measure the content of rosmarinic acid, comprise the following steps:

[0114] 1. Preparation of reference substance solution: same as in Example 1.

[0115] 2. Preparation of the test solution: Weigh about 2.5g of Xiasangju granules, accurately weigh, add 10mL of methanol, weigh, ultrasonicate for 15min, take it out, let it stand, let it cool, make up the weight, filter it through a microporous membrane, and use it as the test solution. Taste.

[0116] 3. Determination by liquid chromatography

[0117] Accurately draw the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for determination.

[0118] Chromatographic conditions:

[0119] Chromatographic column: Agilent Eclipse XDB-C18 (4.6mm×250mm, 5μm);

[0120] Flow rate: 0.5mL.min -1 ;

[0121] Detection wavelength: 325nm;

[0122] Column temperature: 40°C;

[0123] Mobile phase: mobile phase A is acet...

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Abstract

The invention discloses a quality control method for mulberry chrysanthemum granules. The method comprises a step of measuring rosmarinic acid characteristic component content of self-heal by adopting high performance liquid chromatography. The established thin-layer chromatography and high performance liquid chromatography method is simple and convenient and has good practicability; an established fingerprint map for the mulberry chrysanthemum granules is favorable for comprehensively grasping the quality of the mulberry chrysanthemum granules; and meanwhile, the method identifies the main chromatographic peak, makes up the fuzziness of the fingerprint map of the mulberry chrysanthemum granules, jointly forms a quality standard system of the mulberry chrysanthemum granules, and fulfills the purpose of effectively controlling the quality of the mulberry chrysanthemum granules.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a quality control method for Xiasangju granules. Background technique [0002] Xiasangju granule is a characteristic drug in Lingnan area, which is made of selected Prunella vulgaris, mulberry leaves, and wild chrysanthemums, and is refined and processed by modern technology. The 11th category of traditional Chinese medicine, has the effects of clearing the liver and improving eyesight, dispelling wind and heat dissipation, dehumidifying numbness, and relieving sores. It is suitable for treating red eyes, headaches, high blood pressure, dizziness and tinnitus, sore throat, and boils caused by wind-heat and cold. Symptoms of poisoning. [0003] At present, there are 105 domestic and foreign enterprises producing Xiasangju granules. However, in terms of quality standard research, it mainly focuses on qualitative identification. For example, in the "Drug St...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 许招懂林丽美蒋莉娟方铁铮谭新花汝凤
Owner GUANGZHOU XINGQUN PHARMA
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