Method for quickly breeding high-quality multi-resistant rice photo-thermo-sensitive nucleic male sterile line

Abstract

The invention relates to a method for quickly breeding a high-quality multi-resistant rice photo-thermo-sensitive nucleic male sterile line. The method comprises the following steps of: hybridizing a No.1 recessive photo-thermo-sensitive nucleic male sterile line serving as a female parent with No.1 dominant fertile good conventional rice to harvest and plant first-generation hybrid seeds; performing composite hybridization on the first-generation hybrid seeds serving as a male parent and a No.2 recessive photo-thermo-sensitive nucleic male sterile line; harvesting and planting a composite hybridization first generation, selecting fertile plants, and performing anther culture at the monokaryotic stage of pollen before heading and blossoming to obtain stable anther culture plants; selecting photo-thermo-sensitive nucleic male sterile anther culture plants by observing fertility; and performing fertility conversion temperature identification and character screening to obtain the required rice photo-thermo-sensitive nucleic male sterile line. The method integrates technology such as hybridization, composite hybridization, anther culture (microspore culture), character screening and the like, increases genetic background for obtaining excellent descendant materials, improves the probability of obtaining the photo-thermo-sensitive nucleic male sterile line, has the advantages of short period and low cost, and particularly meets the practice requirement of rice breeding in China.

Description

Technical field [0001] The invention relates to a method for cultivating a photothermo-sensitive genic male sterile line of rice, which belongs to the technical field of molecular cell plant breeding, in particular to a fast cultivating method of a high-quality and multi-resistant rice photothermo-sensitive genic male sterile line, which is particularly suitable for starting temperature The rapid cultivation of photo-thermo-sensitive genic male sterile lines with low, strong combining ability and high-quality polyresistance. Background technique [0002] Rice is one of the most important food crops in the world and a staple food for more than half of our population. With the continuous increase of the world’s population, the global arable land area is decreasing year by year, natural disasters occur frequently, and the growth of food is slowing down. Food security has become a hot issue of general concern to the international community. The perennial planting area of ​​hybrid ri...

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects and problems of long breeding cycle, high cost, low flower cultivation efficiency, easy to be affected by environmental and human factors, and low cultivation efficiency in the prior art, and to provide a short breeding cycle, A rapid breeding method for high-quality multi-resistance rice photothermo-sensitive genic male sterile lines with less cost, higher flower cultivation efficiency, less susceptible to environmental and human factors, and higher cultivation efficiency

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