Human hepatocellular carcinoma cell line with portal vein transfer potential

A technology of human liver cancer cell lines and metastatic potential, applied in the field of microbial animal cells, to achieve the effect of enhancing the ability of portal vein tumor thrombus formation

Inactive Publication Date: 2011-01-26
HEPATOBILIARY SURGERY HOSPITAL SECOND MILITARY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on liver cancer cell lines derived from portal vein tumor thrombus of human liver cancer and their establishment methods, and there are no reports on liver cancer cell lines with portal vein metastasis tendency.

Method used

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  • Human hepatocellular carcinoma cell line with portal vein transfer potential
  • Human hepatocellular carcinoma cell line with portal vein transfer potential
  • Human hepatocellular carcinoma cell line with portal vein transfer potential

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] In Example 1, tumor tissue was orthotopically implanted in the liver.

[0071] 4. Experimental animals

[0072] BALB / c-nu / nu nude mice, aged 4-5 weeks, were provided by Shanghai Slack Experimental Animal Center, Chinese Academy of Sciences.

[0073] (2) Method

[0074] The tumor embolus cells were obtained from the orthotopic liver tumor in Example 1, and the tumor thrombus cells were obtained by the digestion method. Through primary culture, tumor "cell islands" (the so-called "cell islands" refer to cell colonies formed by single cell clones, and there are no cells around them. , looks like "islands") clone expansion culture, subculture of tumor cells and other steps to establish a cell line that can be stably passaged in vitro, the specific method is as follows:

[0075] 1. Acquisition and primary culture of tumor thrombus cells

[0076] Remove blood stains and necrotic tissue from fresh nude mouse xenograft tumor tissues, rinse with D-Hank'S containing 5 times co...

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Abstract

The invention discloses a human hepatocellular carcinoma cell line with portal vein transfer potential (CCTCC NO: C200913) and a cell line construction method. After subcutaneous transplantable tumors formed by the cells is inoculated to a nude mouse liver to form tumors, the formation of portal vein cancer emboli can be detected and strong forming ability of portal vein cancer emboli is generated. The cell line construction method comprises the following steps of: (1) transplanting cancer embolus tissues of the human liver cancer portal vein in subcutaneous nude mouse skin; (2) performing passage on transplanted subcutaneous tumors; (3) inoculating the nude mouse liver by using the transplanted subcutaneous tumors of the fifth generation; (4) performing primary culture by using liver neoplasm to construct the line. The construction method has the advantages that: CSQT-2 cells are cultured by taking materials from the human portal vein cancer embolus tissues and transplanting nude mouse liver species in situ, so that the cell line has strong formation ability of portal vein cancer emboli, unparallel advantages in forming mechanism and tendency growing mechanism of the liver cancer portal vein cancer emboli compared with other conventional liver cancer cell lines, and wide application prospect.

Description

technical field [0001] The invention relates to the field of microbial animal cells, in particular to a liver cancer cell line with portal vein metastasis potential. Background technique [0002] Primary liver cancer (HCC) is one of the most common malignant tumors, and the median survival time of untreated patients is less than one year. Surgical resection is currently the most effective treatment for liver cancer. But even if the tumor is removed, most patients still die of postoperative tumor recurrence and metastasis. The main feature of liver cancer metastasis is to grow into the portal vein to form portal vein tumor thrombus. Shanghai Oriental Hepatobiliary Surgery Hospital performed liver tumor resection on 5,524 patients with liver cancer, and the proportion of portal vein tumor thrombi found in the resected specimens reached 67.1% (Chinese Journal of Surgery, 2001, 39: 25-28); The study of growth characteristics proposed that tumor thrombi can be divided into 5 t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12R1/91
Inventor 程树群胡华生王涛程嘉颖谢东吴孟超
Owner HEPATOBILIARY SURGERY HOSPITAL SECOND MILITARY MEDICAL UNIV
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