Kitchen rubbish degrading and eliminating microbial agent, preparation method and fungi thereof

A technology for microbial inoculants and kitchen waste, applied in microorganism-based methods, microorganisms, microorganisms, etc., can solve problems such as increasing sewage treatment pressure, reducing solid waste discharge, and environmental pollution of kitchen waste.

Active Publication Date: 2011-04-13
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology increases the pressure of sewage treatment while reducing the discharge of solid waste. Therefore, it fails to fundamentally solve the environmental pollution problem of kitchen waste

Method used

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  • Kitchen rubbish degrading and eliminating microbial agent, preparation method and fungi thereof
  • Kitchen rubbish degrading and eliminating microbial agent, preparation method and fungi thereof
  • Kitchen rubbish degrading and eliminating microbial agent, preparation method and fungi thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Collection, isolation, screening and identification of Actinomucor elegans No.1:

[0037] 1), collection, separation

[0038] Take 50g of food waste, put it into a 500mL Erlenmeyer flask with 250mL of PDA liquid medium after crushing, and culture it with shaking at 28°C and 120r / min for 24h; Line, cultured at 28°C for 48-72 hours, picked several single colonies with different shapes, each colony was repeatedly streaked on the fungal selective medium plate, until each colony was purified, and several isolates were obtained.

[0039] Preparation of fungal selective medium plate: Peel and cut 200g potatoes into pieces, add 800g water to boil for 0.5h, filter through gauze, add 20g sucrose, 18g agar, add distilled water to 1000mL. At 1.05kg / cm 2 Pressure, sterilize at 121°C for 20 minutes, add penicillin 2000 units / L after cooling, pour plate, add 1-2 drops of 25% lactic acid to spread on the plate.

[0040] Food waste is mainly composed of rice, vegetable lea...

Embodiment 2

[0076] Example 2: Composition of Kitchen Waste Degradation and Elimination Type Microbial Agent II

[0077] Total bacteria count: 2.4×10 10 cfu / mL

[0078] Actinomucor elegans No.1: 1.2×10 10 cfu / mL

[0079] Paenibacillus cineris No. 5: 1.2×10 10 cfu / mL

Embodiment 3

[0080] Example 3: Composition of Kitchen Waste Degradation and Elimination Type Microbial Inoculum II

[0081] Total bacteria count: 1.6×10 11 cfu / mL

[0082] Actinomucor elegans No.1: 1.5×10 11 cfu / mL

[0083] Paenibacillus cineris No.5: 1.2×10 10 cfu / mL

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PUM

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Abstract

The invention discloses actinomucor elegans which has the collection name of No. 1 and the collection number of No. 3881 and is collected in the China General Microbiological Culture Collection Center (CGMCC) located in No.3 first Courtyard Beichen West Road Chaoyang District Beijing City on 1st June 2010. The invention also discloses a kitchen rubbish degrading and eliminating microbial agent II. The Kitchen rubbish degrading and eliminating microbial agent II is a composite liquid microbial agent of 3.0*108-1.5*1011cfu / ml of actinomucor elegans and 3.0*108-1.3*1011cfu / ml of Paenibacilluscineris. The invention also discloses a preparation method of the kitchen rubbish degrading and eliminating microbial agent II. The microbial agent II can be used for degrading kitchen rubbish.

Description

technical field [0001] The invention belongs to the field of domestic waste treatment, and relates to a microbial degradation and elimination technology for kitchen waste, in particular to a microbial antibacterial agent for kitchen waste and a preparation method thereof. Background technique [0002] With the rapid development of my country's social economy, not only the amount of household garbage from urban residents is increasing, but also the proportion of kitchen waste including melon peels, shells, leftovers and leftovers in household garbage is also increasing. Due to the high water content of kitchen waste (90%) and low incineration calorific value (2100-3100kJ / kg), after mixing with other urban waste components, landfill disposal will not only occupy valuable land resources but also produce a large amount of leachate and pollute Underground water system; the use of incineration for power generation disposal cannot meet the calorific value requirements of garbage (t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/00C12N1/20B09B3/00C12R1/01C12R1/645
Inventor 方萍滕一波张允升项福保
Owner ZHEJIANG UNIV
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