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Application of ascites tumor cell in preparing bone cancer pain model

A technology for ascites tumor and bone cancer pain, applied in the biological field, has achieved important academic value and application prospects

Inactive Publication Date: 2011-05-25
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the cell lines cultured in vitro, Walker256 ascites tumor has the advantage of easy cell subculture. So far, there has been no report on using Walker256 ascites tumor cells to make animal calcaneal cancer pain models.

Method used

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  • Application of ascites tumor cell in preparing bone cancer pain model
  • Application of ascites tumor cell in preparing bone cancer pain model
  • Application of ascites tumor cell in preparing bone cancer pain model

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Materials and methods:

[0026] Tumor cells: Walker256 rat breast cancer ascites tumor, provided by Shanghai Institute of Biomedical Engineering;

[0027] Animals: SD female rats, weighing 140-160 grams, provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences;

[0028] Main instruments: von Frey hair (Stoelting, Wood Dale, Illinois, USA); radiant heat pain measuring instrument (Model 390 Paw Stimulator Analgesia Meter, IITC / Life Science Instruments, USA);

[0029] Modeling method:

[0030] Model group: After the rats were anesthetized by intraperitoneal injection of 8% chloral hydrate, the skin of the calcaneus was applied with iodine or 70% alcohol for disinfection, the calcaneus was drilled with a No. , the 4×10 3 ~4×10 5 Walker256 ascites tumor cells were slowly injected into the bone marrow cavity of the tibia. After the injection, iodine was applied to disinfect the needle hole and the surrounding skin, and the tibial pain mode...

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Abstract

The invention belongs to the technical field of biology, and relates to application of a Walker256 ascites tumor cell in preparing a bone cancer pain model. The bone cancer pain model comprises a calcaneus cancer pain or osteitis pain model. In the invention, ethological index tests including radiation heat pain threshold variations, mechanical pain threshold variations and other related indexes are carried out on the prepared calcaneus cancer pain model or osteitis pain model, and the result indicates that the ethological indexes of the experimental animal do not have obvious variations. The bone cancer pain model prepared by the invention provides a valuable experimental research tool and platform for researching the bone cancer pain mechanism, evaluating the analgesic effect of medicines and sieving medicines, simultaneously enriches the types of models of chronic pain experimental animals, and has important academic value and wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of biology and relates to an animal model of cancer pain, in particular to the use of Walker256 ascites tumor cells in preparing a bone cancer pain model. Background technique [0002] Cancer pain is the pain caused by malignant tumors. It is one of the common chronic pathological pains in clinical practice, which seriously affects the quality of life of patients. According to statistics, about 30-50% of tumor patients have mild to moderate pain, and among patients with advanced or metastatic tumors, about 75-95% of patients need severe pain. receive treatment. According to the three-step therapy for cancer pain advocated by the WHO, although clinical practice has achieved certain results, due to the lack of understanding of the mechanism of cancer pain, and various drug therapies, radiotherapy, chemotherapy and other therapies have many side effects as follows, Such as: addiction, tolerance, immunosuppres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61P19/08A61P35/00A61P29/00A61K35/13
Inventor 毛应启梁王彦青
Owner FUDAN UNIV
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