Method for realizing high concentration alcoholic fermentation through completely saccharified high-sugar fed-batch
An alcohol fermentation, high-concentration technology, applied in the direction of fermentation, biofuels, etc., can solve the problems of fermentation difficulties, prolonged fermentation time, and limitation of alcohol fermentation rate, etc., to achieve the goal of releasing substrate inhibition, increasing alcohol yield, and increasing alcohol concentration Effect
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Embodiment 1
[0018] Embodiment 1: A method of fully saccharification and high-sugar fed-batch to realize high-concentration alcohol fermentation adopts the following process steps:
[0019] 1. The cassava is crushed and passed through a 40-mesh sieve.
[0020] 2. Weigh 2120kg, add 3392L of tap water according to the solid-to-liquid ratio of 1:1.6, adjust the pH to 6.0, and add 10U / g cassava according to the amount of liquefaction enzyme, then heat up to above 80°C for liquefaction, after the iodine test is complete Lower the temperature to 60°C, adjust the pH to 5.0 and add glucoamylase for saccharification. The amount of glucoamylase added is 130U / g cassava. After the saccharification is complete, the volume is 5000L.
[0021] 3. Take out 2000L of the saccharification liquid, dilute it with water to 4200L, put it into the fermenter, and insert the yeast seed liquid to start the fermentation. The volume of the seed solution is 10% of the volume of the diluted whole sugar solution, and th...
Embodiment 2
[0023] Embodiment 2: A method of fully saccharification and high-sugar fed-batch to realize high-concentration alcohol fermentation adopts the following process steps:
[0024] 1. Weigh 1690g of cornstarch;
[0025] 2. Add 2704L of tap water as mixing material according to the solid-to-liquid ratio of 1:1.6. After liquefaction and saccharification (the type of enzyme and the amount added are the same as in the example), the volume of the saccharification solution is 3600L;
[0026] 3. Take out 720L of the saccharification liquid, dilute it with water to 3600L, flow into the fermenter, and insert yeast seed liquid to start fermentation. The volume of the seed solution is 10% of the volume of the diluted whole sugar solution, and the fermentation temperature is controlled at 32°C.
[0027] 4. When the fermentation progresses to 10 hours, the remaining 2880L saccharification liquid is flowed into the fermenter at a flow rate of 58L / h. After the feeding is completed, the fermenta...
Embodiment 3
[0028] Embodiment 3: A method of fully saccharification and high-sugar fed-batch to realize high-concentration alcohol fermentation, adopting the following process steps:
[0029] 1. Crush cassava and pass through a 40-mesh sieve;
[0030] 2. Weigh 2000g of cassava flour and add 3900L of tap water as mixing material at a solid-to-liquid ratio of 1:1.8. After liquefaction and saccharification (the enzyme type and addition amount are the same as those in the examples), the volume of the saccharification solution is 4500L;
[0031] 3. Take out 1800L of the saccharified liquid, dilute it with water to 4300L, flow into the fermenter, and insert yeast seed liquid to start fermentation. The volume of the seed solution is 10% of the volume of the diluted whole sugar solution, and the fermentation temperature is controlled at 30°C.
[0032] 4. When the fermentation progresses to 10 hours, the remaining 2700L saccharification liquid is flowed into the fermenter at a flow rate of 55L / h....
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