Pine pollen aqueous extract for treating premature ovarian failure of female as well as preparation method and application thereof

Abstract

The invention provides a pine pollen aqueous extract for treating premature ovarian failure of the females as well as a preparation method and an application thereof, relating to the technical field of extracting effective substances from the pine pollen. The preparation method comprises the following steps: the pine pollen is subjected to wall breaking at a low temperature, and is extracted with ultrasonic after the pine pollen is immersed in normal-pressure normal-temperature water; solid impurities are removed to select water-soluble substances in the pine pollen; and effective components with the molecular weight lower than 5,000D is ultra filtered and separated at the environmental temperature of between 1 and 5 DEG C to obtain the gymnosperm pine pollen aqueous extract for treating the premature ovarian failure. The pine pollen aqueous extract can be used for preparing an oral preparation for treating the premature ovarian failure, has a good absorption effect and low price; and the pine pollen aqueous extract can be used for preparing an injection preparation for treating the premature ovarian failure, which can be absorbed quickly, has strong treatment pertinence, can be used for general health-care of the females, and prevents occurrence of the premature ovarian failure.

Description

technical field [0001] The invention relates to the technical field of water extraction of effective substances from plants, especially gymnosperm pine pollen. Background technique [0002] Premature ovarian failure (POF) refers to the failure of ovarian function in women with normal development before the age of 40 for some reason. It is manifested as secondary amenorrhea, low estrogen and high gonadotropin state, which is customarily called early menopause. The incidence rate of premature ovarian failure before the age of 40 is about 1% to 3%, while the incidence rate within the age of 30 is 0.1%. Current studies suggest that the reserve of primordial germ cells is a key factor in determining the length of a woman's reproductive life, and the formation of primordial germ cells is controlled by multiple genes. In addition to being regulated by innate genetic factors, the maintenance of ovarian function in mature women is also affected by a variety of acquired factors. So ...

AI Technical Summary

Problems solved by technology

Pine pollen is rich in proteins, amino acids, unsaturated fatty acids, polysaccharides, vitamins, dietary fiber, enzymes, flavonoids, nucleic acids, natural hormones and other nutritional components and biologically active substances. Numerous studies have shown that gymnosperm pine pollen can regulate hormones At the receptor level, it exerts the pharmacological effects of estrogen-like and anti-androgen, and makes the premature ovarian reversal nearly normal, but there are not many studies on what substances in pine pollen work.

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