Assay kit for diagnosis of hypertensive disorders of pregnancy

Abstract

The invention belongs to the field of biotechnologies and relates to an assay kit for diagnosis of hypertensive disorders of pregnancy. The assay kit comprises specific antibodies respectively aiming at Kertin, Alpha-actinin-4, Afamin, Alpha-2-macroglobulin, ALB, SERPINA1, Vimentin, Tubulin beta-2C chain and / or angiotensinogen protein. The assay kit can detect multiple proteins which are expressed differentially and are in urine samples of patients suffering from hypertensive disorders of pregnancy. Information of the above multiple proteins expressed differentially can be as a basis for the research of pathogenesis of preeclampsia nephropathy proteinuria, and provides a clue for the search of biomarkers utilized for an early diagnosis and a forecast of preeclampsia nephropathy. The assay kit provided by the invention is a non-invasive detecting tool of biomarkers of hypertensive disorders of pregnancy, and can be utilized for diagnosing preeclampsia nephropathy and hypertensive disorders of pregnancy.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit for diagnosing pregnancy-induced hypertension. Background technique [0002] Hypertensive disorder of pregnancy is one of the most common syndromes associated with pregnancy, which affects 7-10% of pregnant women, mainly including gestational hypertension and preeclampsia; preeclampsia, also known as preeclampsia, is pregnancy-induced hypertension and The main cause of massive proteinuria, its clinical features are hypertension, edema, massive proteinuria, and renal insufficiency after 20 weeks of pregnancy, and gradually aggravated with the increase of pregnancy, and the condition often improves rapidly after delivery, mostly in postpartum 6 Return to normal within a week, no later than 3 months. In 2001, Williams regarded proteinuria as one of the necessary conditions for the diagnosis of preeclampsia, indicating the prevalence of renal damage. Proteinuria is an objective ind...

AI Technical Summary

Problems solved by technology

[0005] At present, the classic two-dimensional electrophoresis (2-DE) has been widely used in the study of urine proteomics, but its shortcomings are gradually recognized, such as low sensitivity, time-consuming, and often miss some low-abundance proteins

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