Testing kit and testing method for early screening or assisting diagnosis of urinary system diseases as well as applications of testing kit and testing method

A technique for urinary system diseases and detection kits is applied in the field of detection kits for urinary system disease markers, which can solve the problems that protein markers are not widely used clinically and the source of urine protein is complex, and achieves unique and large detection methods. Market prospects, highly specific effects

Inactive Publication Date: 2012-07-18
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the source of urine protein is very complex, and is affected by factors such as environmental diet, physiological fluctuations are very

Method used

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  • Testing kit and testing method for early screening or assisting diagnosis of urinary system diseases as well as applications of testing kit and testing method
  • Testing kit and testing method for early screening or assisting diagnosis of urinary system diseases as well as applications of testing kit and testing method
  • Testing kit and testing method for early screening or assisting diagnosis of urinary system diseases as well as applications of testing kit and testing method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0140] Example 1

[0141] This example illustrates that urine is selected as the research object for finding stable urine protein markers in healthy people, and the stable protein markers in urine of healthy people are searched for as indicators for early screening of urinary system diseases.

[0142] 1. Experimental method

[0143] 1. Screening of healthy donors

[0144] Select 100 healthy men and 100 healthy women from Chongqing Third Military Medical University. See Table 1 for specific physical examination indicators.

[0145] Table I

[0146]

[0147] 2. Specimen collection

[0148] Urine specimens were collected every two months for a total of 3 times. Collect 50ml of mid-morning urine at 8:00 am each time, centrifuge at 3000 rpm / min for 20 minutes at 4°C, save the supernatant, and store at 4°C.

[0149] 3. Urine treatment

[0150] Immediately after urine collection, a small protease inhibitor cocktail (CompleteTM; Roche Diagnostics, Mannheim, Germany) was added to every 50ml urine...

Example Embodiment

[0184] Example 2

[0185] This example provides multiple embodiments of the kit of the present invention. Those skilled in the art can modify these embodiments according to the following exemplified embodiments, combined with the teachings of the present invention, and the scope of the present invention also includes these modifications. implementation plan.

[0186] For ease of description, in Table 6 below, unless otherwise specified, the capture reagents for the antibody antigen binding mode are all monoclonal antibodies as examples; the receptor ligand binding mode is for high-affinity receptors or ligands. At the same time, to simplify the description, the kits listed in Table 6 only list the main components, such as capture reagents, detection reagents, detectable labeled molecules, etc., while other optional components such as diluents, buffers, and cleaning agents are omitted. At the same time, the following descriptions and tables only give the detection methods of detect...

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Abstract

The invention relates to a testing kit and a testing method for early screening or assisting diagnosis of urinary system diseases. The testing kit contains at least one testing reagent and a capturing reagent and can be used for specifically recognizing and correctly quantifying any one or combination of more proteins of COL6A1, GC, RBP4, DNASE1, GAA, SERPING1, CTSD, KNG1, UMOD, PIGR, AHSG, A1BG, AMBP, AZGP1, PTGDS, HPX, CD14 and ORM1 which are stably expressed in the urine of healthy people. The testing method can test whether the content of any of the eighteen proteins in the urine of a person to be tested is abnormal so as to prompt the generation of the urinary system diseases and achieve the purpose of early screening or assisting the diagnosis of the urinary system diseases.

Description

technical field [0001] The invention relates to a detection kit and a detection method for early screening or auxiliary diagnosis of urinary system diseases or pathological conditions. Specifically, the present invention relates to the screening of 18 marker candidate proteins stably expressed in the urine of healthy people, their use, a detection kit containing them as urinary system disease markers, and a detection method thereof. Background technique [0002] It is of great significance to detect or assist in the diagnosis of urinary system diseases through early screening, especially malignant diseases of the urinary system such as tumors, etc., for the prevention and cure of diseases. The source of urine is simple, convenient and non-invasive, and is easily accepted by patients without discomfort or contraindications. Urine can reflect the overall condition of the kidney, and can more timely and accurately reflect the physiological state and functional level of the kid...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 贺伟峰吴军罗高兴王颖
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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