Culture solution continuous filling method and system for chlamydomonas culture

Abstract

The present invention relates to a method and system for continuously filling culture solution for the cultivation of unicellular algae. The method includes heating, disinfecting and cooling seawater, storing it, mixing it with the culture solution containing nutrient salt in each nutrient salt tank, and passing it through a sand filter device. Filtration; finally through the pipeline with quantitative control clamp into the single cell algae culture reactor. Its system includes a constant pressure storage tank with an infusion pipeline connected with a seawater heating and disinfection device, and a nutrient salt tank that injects nutrient solution into the infusion pipeline through a pump; The branch of the delivery pipeline communicates with the hanging bag photobioreactor. The method of the invention can realize the automatic, aseptic, quantitative and unattended flow operation of the culture medium. The system of the invention has airtightness, systematicness, quantification, cleaning function and buffering effect.

Description

technical field [0001] The present invention relates to a method and system for continuously filling culture fluid for algae cultivation, in particular to a method and system for continuously filling culture fluid for cultivating unicellular algae, which is suitable for continuous filling culture when cultivating unicellular algae liquid. Background technique [0002] The cultivation of algae, especially the single-celled algae, has not yet formed a scale, and most of them stay in the flask cultivation in the laboratory. During the flask cultivation process, the feed liquid is filled by using the laboratory container. There is also a small amount of culture that simply takes seawater from the sea with a bucket or other container, pours it into an open container such as a pot or bucket, and cultivates it by using an air stone to inflate it. In production, there are also a small number of earthen ponds or cement ponds, which are cultivated in the open air after adding seawate...

AI Technical Summary

Problems solved by technology

[0003] The disadvantage of the above method is that the seawater added is generally not sterilized and not filtered effectively, so it is easy to bring in impurities or bacteria, or even harmful organisms.

When adding the feed liquid in this way, the process of liquid addition is discontinuous, which will cause the instability of the culture water environment

When a large amount of water is added at one time, it will cause a greater impact on the temperature and salinity of the culture water body. Sudden changes in the culture water environment will cause a stress response or even stop growth of the unicellular algae

Quantitative control cannot be achieved when adding the feed liquid in this way

[0004] The reason for the above problems is that the existing methods for cultivating Unicellular algae remain in the laboratory stage, and the cultivation method of Unicellular algae is relatively backward, and no effective production mode has been formed. The link has not been paid attention to, and the process of filling the material liquid is also relatively backward

Therefore, when the cultivation of unicellular algae is moving towards production and batch production, and the flow operation is carried out, the existing feed liquid filling method cannot meet the requirements

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