Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying gray mold resistance of gerbera

A technology for resistance identification and botrytis cinerea, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and determination/inspection of microorganisms. To achieve the effect of accurate identification and promotion of gerbera botrytis resistance breeding

Inactive Publication Date: 2012-11-28
YUNNAN AGRICULTURAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the deficiencies and deficiencies that the field live plant inoculation method of the prior art has the defects and deficiencies of test plant infection, long identification period, and easily affected by environmental conditions to the identification of gerbera gray mold resistance. The purpose is to provide a simple, rapid, accurate and large-scale method for screening and identifying gerbera botrytis resistance resources, providing support for further cross breeding or molecular marker assisted breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying gray mold resistance of gerbera
  • Method for identifying gray mold resistance of gerbera
  • Method for identifying gray mold resistance of gerbera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Taking petals as the embodiment of inoculation material

[0041] (1) Plant material

[0042] The gerbera cut-flower cultivars tested with different resistance to Botrytis cinerea were: Macy, Kaliki, Risto, Serena, CK100, 3142, 5544, a total of 7 genotypes, provided by Dutch gerbera breeding company Florist and Shreurs, and fresh petals were collected as Inoculation material;

[0043] (2) Botrytis cinerea Botrytis cinerea Culture and preparation of conidia suspension

[0044] Botrytis cinerea was cultivated with potato dextrose agar medium (abbreviation: PDA medium) Botrytis cinerea , Botrytis cinerea ( Botrytis cinerea) inoculated in the center of a 10cm-diameter petri dish with potato dextrose agar medium on a superbacteria workbench, and cultivated under continuous light for 16 hours at a temperature of 20°C to 22°C and a light intensity of 1800lx. After 3 days of cultivation, it was transferred to dark conditions for cultivation After 7 days, afte...

Embodiment 2

[0063] Example 2 Take the embodiment of inoculation material with intact young leaves

[0064] (1) Plant material

[0065] The gerbera cut flower cultivar and source for testing are the same as in Example 1, and the fresh complete young leaves are collected as inoculation material;

[0066] (2) Botrytis cinerea Botrytis cinerea Culture and preparation of conidia suspension

[0067] With embodiment 1.

[0068] (3) Preparation of inoculum

[0069] With embodiment 1.

[0070] (4) Inoculation

[0071] Inoculate 2 μl of the inoculum solution containing different concentrations of conidia and PD prepared in step (3) onto the young leaves described in step (1), and keep the young leaves in a plastic film, with a relative humidity of 90% to 95 %, under the condition of light intensity of 1800lx and temperature of 20℃~22℃, the disease development was observed 24 hours after inoculation, and the disease resistance of the tested materials was judged according to the dise...

Embodiment 3

[0086] Example 3 Test of suitable inoculation material

[0087] Except that the inoculation material described in Example 1 was tested, Example 2 also tested the gerbera leaf block and stem section, and the test method was carried out using the treatment method in Example 1.

[0088] (1) Plant material

[0089] The gerbera cut-flower cultivars tested with different resistance to Botrytis cinerea were: Macy, Kaliki, Risto, Serena, CK100, 3142, 5544, a total of 7 genotypes, provided by Dutch gerbera breeding companies Florist and Shreurs, which were fresh Collected gerbera leaf pieces and stem sections are used as inoculation materials. The leaf pieces have three types of diameters: 1.5cm, 2.5cm, and 3.5cm, and the length of the stem section is 8cm.

[0090] (2) Botrytis cinerea Botrytis cinerea Culture and preparation of conidia suspension

[0091] Botrytis cinerea was cultivated with potato dextrose agar medium (abbreviation: PDA medium) Botrytis cinerea , Inoculate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying gray mold resistance of gerbera, belonging to the technical field of plant disease resistance identification. The method comprises the following steps: (1) selection of proper inoculation material: selecting freshly collected and integrated young leaves and petals as the inoculation material; (2) culture of inoculation material and preparation of conidia suspension; (3) preparation of inoculation liquid: before inoculation, diluting the conidia stock solution into an inoculation liquid containing 3 wt% of potato glucose and 1*10<6> / ml conidia, or into an inoculation liquid containing 6wt% of potato glucose and 3*10<5> / ml conidia; and (4) inoculation. The method disclosed by the invention is carried out under manually controlled stable conditions, and achieves the goal of identifying the gray mold resistance of gerbera in a simple, quick and accurate way; and the accuracy of the identification result is high.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, and in particular relates to a method for in vitro resistance identification of gerbera gray mold. Background technique [0002] Gerbera ( Gerbera spp .) is one of the five most important cut flowers in the world, and the application of potted flowers is becoming more and more popular, which has important economic value. Botrytis ( Botrytis cinerea ) is an air-borne saprophytic fungus with a wide suitable temperature range (4°C-25°C) and infects more than 200 kinds of hosts. Especially in the post-harvest storage and transportation of cut gerbera flowers, environmental conditions such as high humidity and large temperature fluctuations produce condensation, which provides good conditions for the growth and reproduction of Botrytis cinerea, and soft rot caused by Botrytis cinerea has become a One of the limiting factors for post-harvest quality maintenance of cu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18C12R1/645
Inventor 吴红芝吴景芝吴兴恩陈溪都婷
Owner YUNNAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com