Culture medium preparation device and method
A medium preparation and culture medium technology, which is applied in the medium preparation device and the field of preparation, can solve the problems of affecting accuracy, difficulty in control, loss of medium components, etc., and achieve the effect of high preparation efficiency, high precision and simple operation
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Embodiment 1
[0018] Embodiment 1 culture medium preparation device
[0019] Such as figure 1 As shown, the culture medium preparation device of the present invention comprises a preparation container 2, a stirring device 11, a support 12, a hollow hose 3 with openings at both ends; the preparation container 2 is placed on the stirring device 11, and the middle part of the hollow hose 3 is fixed on On the support 12, one end of the hollow hose 3, namely the inlet end, is inserted into the bottom of the preparation container 2 with the culture medium, and the other end, namely the outlet end, extends to the lower part of the opening of the culture container 4.
[0020] In this embodiment: the preparation container 2 is a beaker; the stirring device 11 and the support 12 are two components of the magnetic stirrer 1; position; the hollow hose 3 is a rubber hose with a length of 1.2m.
[0021] When the vertical distance between the bottom of the preparation container 2 and the operating platf...
Embodiment 2
[0022] Embodiment 2 culture medium preparation method
[0023] Taking the preparation of MS+1.0mg / L6-BA solid medium as an example, the medium preparation method using the preparation device of the preferred embodiment 1 of the present invention comprises the following steps:
[0024] 1) Add all the required macroelements, trace elements, organic elements, hormones, sucrose and deionized water into a 2L preparation container beaker;
[0025] 2) Place the beaker on a magnetic stirrer and stir until the sucrose dissolves, set the volume to 2L, and adjust the pH value to 5.8;
[0026] 3) Add 7g of agar powder and keep stirring;
[0027] 4) Pass the rubber hose through the arc-shaped device on the bracket of the magnetic stirrer, so that the middle part of the hose is fixed on the bracket to prevent the hose from moving and discounting;
[0028] 5) Put the end of the rubber hose as the inlet end into the bottom of the beaker;
[0029] 6) Place 60 triangular flasks with a capaci...
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