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Biological tissue fixing agent and preparation method thereof

A biological tissue and fixative technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve problems such as inconvenient portability, remote mailing, easy leakage, and insufficient fixation, so as to achieve a well-preserved tissue structure , low cost, easy to carry

Inactive Publication Date: 2011-11-23
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, formalin is in a liquid state, which is inconvenient to carry and long-distance mail, and it is easy to leak in an airtight container, and tissue floating or sticking to the wall may cause insufficient fixation when the tissue is placed in it.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] First, take 10 g of water and heat it to boiling, add 1 g of agar and keep stirring until it is completely dissolved, then stop heating, add 83.8 g of water, add 2.5 g of formaldehyde solution with a mass fraction of 40%, NaH 2 PO 4 0.2g, Na 2 HPO 4 2 g and 0.5 g of glycerin, stirred evenly until completely cooled, to prepare the biological tissue fixative of this embodiment.

Embodiment 2

[0030] First, take 20g of water and heat it to boiling, add 3g of agar and keep stirring until it is completely dissolved, then stop heating, add 51g of water, add 20g of formaldehyde solution with a mass fraction of 35%, NaH 2 PO 4 0.5g, Na 2 HPO 4 1.5 g and 4 g of glycerin were stirred evenly until completely cooled to prepare the biological tissue fixative of this embodiment.

Embodiment 3

[0032] First, take 26g of water and heat it to boiling, add 5g of agar and keep stirring until it is completely dissolved, then stop heating, add 54.5g of water, add 10g of formaldehyde solution with a mass fraction of 35%, NaH 2 PO 4 0.5g, Na 2 HPO 4 1 g and 3 g of glycerin, stirred evenly until completely cooled, to prepare the biological tissue fixative of this embodiment.

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PUM

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Abstract

The invention discloses a biological tissue fixing agent and a preparation method thereof. The biological tissue fixing agent comprises the following components in percentage by weight: 1-10% of formaldehyde, 1-10% of agar, 0.5-5% of glycerol, 0.2-1% of NaH2PO4, 0.5-2% of Na2HPO4 and the balance of water. The biological tissue fixing agent disclosed by the invention is gelatinous, overcomes the defects of a liquid fixing agent, is convenient in carrying and long-distance mailing, can not leak in a non-tight container, can not cause insufficient fixation resulting from tissue floatation or adherence or the like when tissues are put in the biological tissue fixing agent, is used for fixing needle biopsy tissues, can keep perfect tissue structures and clear cellular morphology, and has a good fixing effect. In the invention, the preparation method is simple, the cost is low, and the period of validity lasts more than one year.

Description

technical field [0001] The invention relates to a fixative composition for preserving tissues and biological samples and a preparation method thereof. Background technique [0002] After the tissue specimens are isolated, they are fixed with a fixative and made into pathological sections for medical pathological diagnosis. The most commonly used fixative in hospital pathology departments and surgical operating rooms is formalin, which is used to fix pathological tissue specimens to prevent tissue autolysis, structural destruction, and cell degeneration. However, formalin is in a liquid state, which is inconvenient to carry and long-distance mail, and it is easy to leak in an airtight container, and tissue floating or sticking to the wall may cause insufficient fixation when the tissue is placed in it. Contents of the invention [0003] In view of this, the object of the present invention is to provide a biological tissue fixative and a preparation method thereof. The fixa...

Claims

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Application Information

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IPC IPC(8): A01N1/00A01N1/02
Inventor 詹俊何娅妮
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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