Granulocyte separating medium, and method for granulocyte separating and activity detecting

A technology for measuring activity and granulocytes, which is applied in the field of biomedicine to achieve the effect of improving the effect

Inactive Publication Date: 2012-01-18
深圳市中美康士生物科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a granulocyte separation liquid and its granulocyte separation and activity assay method, aiming at solving the problem of how to separate and screen highly efficient granulocytes

Method used

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  • Granulocyte separating medium, and method for granulocyte separating and activity detecting
  • Granulocyte separating medium, and method for granulocyte separating and activity detecting
  • Granulocyte separating medium, and method for granulocyte separating and activity detecting

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preparation example Construction

[0051] The preparation method of the granulocyte separation liquid with different concentration gradients is as follows:

[0052] S1. Take n (n is a natural number greater than 1) 50mL sterile centrifuge tubes and mark them as 1,...,n respectively, corresponding to granulocyte separation solution 1-n.

[0053] S2. Put the centrifuge tubes on the balance and weigh them respectively, and record the weight of each tube.

[0054] S3. According to the ratio and required density, add granulocyte separation mother solution C and B to No. 1-n centrifuge tubes respectively, and shake gently; then add granulocyte separation mother solution A respectively, and mix well with a pipette.

[0055] S4. Put the centrifuge tubes on the balance and weigh them separately, subtract the weight of each empty tube respectively, and compare with the corresponding weight in the table. If there is any error, add mother liquor A or C to adjust until the weight matches. .

[0056] S5. Use a 0.45 μm ster...

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Abstract

The invention discloses a granulocyte separating medium, and a method for granulocyte separating and activity detecting by using the granulocyte separating medium. The granulocyte separating medium is prepared from percoll, a 10* phosphate buffer solution and physiological saline, wherein a volume ratio of the percoll to the 10* phosphate buffer solution to the physiological saline is 3-8:1:1-5. With the present invention, the special granulocyte separating medium is adopted to separate the granulocyte, and then detect the activity of the granulocyte so as to screen the high-performance granulocyte, improve the tumor therapy effect of the granulocyte, and easily realize targeted selection of the donor.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for separating and measuring the activity of granulocytes. Background technique [0002] Tumor is a major disease that endangers human life and health, and it has been listed as the second cause of death in the population. According to the statistics of the World Health Organization, there are 9 million new cancers and 5 million deaths worldwide every year. The current treatment methods for tumors are mainly surgery, radiotherapy and chemotherapy. Among them, surgical treatment is only suitable for early-stage tumors, while radiotherapy and chemotherapy are more toxic and side effects. Therefore, developing a new generation of safe and effective anti-tumor biological therapy will have extremely important social and economic benefits. Allogeneic human granulocyte infusion therapy is to inject cancer killer activity (cancer killer activity, CKA) granulocytes from healthy dono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0787C12Q1/02G01N21/31
Inventor 李晓祥
Owner 深圳市中美康士生物科技有限公司
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