Bcl11a siRNA-2292 restraining expression of BCL11A and proliferation of tumorous B cells and application thereof

A technology of bcl11asiRNA-2292 and bcl-2siRNA, which is applied in the direction of DNA/RNA fragments, antineoplastic drugs, recombinant DNA technology, etc., can solve the problems of lack of Bcl11asiRNA, etc., and achieve the effect of huge market potential

Inactive Publication Date: 2012-02-15
JINAN UNIVERSITY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing studies have shown that BCL11A may be associated with the occurrence of B-cell tumors, but there is no evidence that Bcl11a siRNA can be used in tumors, especially in the treatment and / or prevention of B-cell tumors

Method used

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  • Bcl11a siRNA-2292 restraining expression of BCL11A and proliferation of tumorous B cells and application thereof
  • Bcl11a siRNA-2292 restraining expression of BCL11A and proliferation of tumorous B cells and application thereof
  • Bcl11a siRNA-2292 restraining expression of BCL11A and proliferation of tumorous B cells and application thereof

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Embodiment 1

[0035] 1. Experimental materials and methods

[0036] (1) Design and synthesis of Bcl11a siRNA

[0037] According to the design tool software provided by Ambion on the Internet (see www.ambion.com / techlib / misc / siRNA finder.html), three siRNA sequences were designed for the Bcl11a gene, named Bcl11a siRNA-2292, Bcl11a siRNA-475 and Bcl11a respectively siRNA-319, negative siRNA labeled with green fluorescent FAM at the 5' end of the positive sense strand and siRNA with irrelevant sequence were designed at the same time as a control, which was synthesized by Shanghai Jikai Gene Chemical Technology Co., Ltd.

[0038] The sequence of Bcl11a siRNA-2292 is as follows:

[0039] The sequence of the sense strand is: 5'-CGGGCGAAAGGCCUUAUAAUU-3';

[0040] The sequence of the antisense strand is: 5'-UUAUAAGGCCUUUCGCCCGUG-3';

[0041] The sequence of Bcl11a siRNA-475 is as follows:

[0042] Sense strand: 5'-GCAGGGUAUUUGUAAAGAUUU-3';

[0043] Antisense strand: 5'-AUCUUUACAAAUACCCUGCGG-3';...

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Abstract

The invention discloses a Bcl11a siRNA-2292 restraining the expression of BCL11A and the proliferation of tumorous B cells, which has the sense strand sequence of 5'-CGGGCGAAAGG CCUUAUAAUU-3' and the antisense strand sequence of 5'-UUAUAAGGCCUUU CGCCCGUG-3'. The Bcl11a siRNA-2292 can efficiently restrain the expression of the BCL11A, effectively restrain the proliferation of the tumorous B cells and promote the apoptosis of the tumorous B cells. The invention also confirms that the combination of the Bcl11a siRNA-2292 with the Bcl-2siRNA can more effectively restrain the proliferation and promote the apoptosis of the tumorous B cells. The Bcl11a siRNA-2292 restraining the expression of the BCL11A and the proliferation of the tumorous B cells has important meanings in developing novel anti-B cell oncogene medicines and improving the treatment effect of B cell tumors.

Description

technical field [0001] The invention relates to the field of siRNA and its application, in particular to a Bcl11a siRNA-2292 inhibiting the expression of BCL11A and the proliferation of tumorous B cells and its application in the preparation of drugs for treating and / or preventing B cell tumors. Background technique [0002] RNAi technology is a new way to inhibit gene expression with high efficiency and high specificity. In vivo and in vitro studies have shown that RNAi technology has better application prospects than antisense nucleic acid in cancer gene therapy. A large number of studies have shown that inhibiting the expression of tumor-related genes by RNAi in vitro can inhibit the proliferation and growth of tumor cells, induce apoptosis, and increase the sensitivity of tumor cells to drugs. [0003] The Bcl11a (B-cell lymphoma / leukemia 11A) gene is located at 2p16.1 and is a krüppel-like zinc finger gene belonging to the BCL11 family (including BCL11A and BCL11B). T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/02
Inventor 何冬梅高杨军李扬秋
Owner JINAN UNIVERSITY
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