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Tumor marker and use thereof

A tumor marker and nipple technology, applied in the field of tumor markers and their utilization, can solve the problems of difficulty in judging primary lesions and lymph node metastases and other problems.

Inactive Publication Date: 2012-02-29
LSIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to thyroid papillary carcinoma, there are many other malignancies that present papillary structures, so it is not only difficult to judge whether the lesion is primary, but also difficult to judge neoplastic lesions with lymph node metastasis

Method used

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  • Tumor marker and use thereof
  • Tumor marker and use thereof
  • Tumor marker and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0070] [1. Acquisition of human SNX5 cDNA]

[0071] Total RNA extracted from human HacaT cells was used as a template, and a forward primer (pCMV-HA-SNX5 FW: 5′-CAGGCCCGAATTCGGATGGCCGCGGTTCCCGAG-3′ (SEQ ID NO. 4)) and a reverse primer (pCMV-HA-SNX5 RV: 5 '-GATCTCGGTCGACCGTGAAGGCATATCAGTTAT-3' (SEQ ID NO: 5)) to perform RT-PCR, thereby obtaining human SNX5 cDNA. The obtained human SNX5 cDNA was inserted into the expression vector pET3c (Novagen) for Escherichia coli and the expression vector pCMV-HA (BD Bioscience) for mammals to prepare pET3c-SNX5 and pCMV-HA-SNX5, respectively. Here, pCMV-HA-SNX5 FW is a primer in which a restriction enzyme site and the like are linked to the sequence of the 5' region of human SNX5 cDNA (ATGGCCGCGGTTCCCGAG (SEQ ID NO: 6)), and pCMV-HA-SNX5 RV is a primer of the 3' region of human SNX5 cDNA. A primer for linking a restriction enzyme site or the like to the complementary sequence of the 'region sequence (ataactgatatgccttcac (SEQ ID NO: 7)).

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Abstract

SNX5 is detected in a sample collected from a subject. SNX5 can be used as a tumor marker specific to papillary thyroid carcinoma, whereby the diagnosis of papillary thyroid carcinoma can be achieved readily. A technique for detecting papillary thyroid carcinoma using the tumor marker is also provided.

Description

technical field [0001] The present invention relates to a tumor marker and its utilization, more specifically, to a method for detecting a tumor marker suitable for diagnosing thyroid papillary carcinoma and a tool used in the method. Background technique [0002] Papillary thyroid carcinoma is the most frequent malignancy among thyroid cancers and may have a poor prognosis when it metastasizes to the lung or neck lymph nodes. Therefore, the diagnosis of thyroid papillary carcinoma is very important. For the diagnosis of thyroid papillary carcinoma, histopathological research is indispensable. However, in addition to thyroid papillary carcinoma, there are many other malignant tumors that appear as papillary structures, so it is difficult not only to judge whether the lesion is primary, but also to judge the neoplastic lesion with lymph node metastasis. [0003] Histopathologic characterization of papillary thyroid carcinoma was evaluated based on the expression pattern of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574C12Q1/68G01N33/53C12N15/09
CPCC12Q1/6886C12Q2600/112G01N33/57407G01N2800/046
Inventor 一宫慎吾菊地智树外冈晓子佐藤昇志
Owner LSIP
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