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Polymerase chain reaction based chiral gold dimer controllable assembly method

A technology of gold dimer, assembly method, applied in the field of material chemistry

Inactive Publication Date: 2012-03-21
王利兵 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the assembly of gold nanoparticle dimers by polymerase chain reaction is rarely reported, and it is worth noting that there are very few studies on the chiral properties of gold nanoparticle dimers.

Method used

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  • Polymerase chain reaction based chiral gold dimer controllable assembly method
  • Polymerase chain reaction based chiral gold dimer controllable assembly method
  • Polymerase chain reaction based chiral gold dimer controllable assembly method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the assembly of 18nm gold dimer with particle size

[0042] (1) Synthesis of gold nanoparticles

[0043] The synthesis scheme of gold nanoparticles is as follows: add 95mL of water to a clean Erlenmeyer flask, then add 5mL of chloroauric acid with a concentration of 2g / L to the water, heat, boil, and then add 2.5mL of 1% trisodium citrate Solution, stirring while heating, the solution color changes from light yellow to red, and the reaction continues for 6-8min so that the trisodium citrate can completely settle. Finally, the solution was cooled to room temperature, diluted to 100 mL, and stored at 4°C. That is, 18nm gold nanoparticles were produced.

[0044] (2) Coupling of gold nanoparticles and primers

[0045] Centrifuge 1 mL of prepared gold nanoparticles at 10,000 r / min for 10 min, disperse with 1 mL of water, centrifuge at 10,000 r / min for 10 min, discard the supernatant, and disperse with 100 μL of 0.01M PBS with pH 8.0, 0.01% SDS, at 4°C Pr...

Embodiment 2

[0055] The assembly of embodiment 2 10nm and 25nm different size gold dimers

[0056] Adjust the amount of citric acid to obtain gold nanoparticles with different particle sizes, reduce the amount of citric acid to 1.4mL to get larger gold nanoparticles of 25nm, increase the amount of citric acid to 5.0mL to get smaller gold nanoparticles of about 10nm particle. The 25nm gold nanoparticles are coupled to the upstream primers, while the 10nm gold nanoparticles are coupled to the downstream primers. Other steps are with embodiment 1.

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Abstract

A polymerase chain reaction based chiral gold dimer controllable assembly method belongs to the technical field of material chemistry. The invention has the following main implementation steps of: (1) preparing gold nanoparticles with different particle sizes; (2) coupling gold nanoparticles with upstream and downstream primers; (3) modifying gold nanoparticle-primer conjugates; (4) performing PCR by the use of the obtained gold nanoparticle-primer conjugates, representing an assembly structure by TEM; (5) carrying out CD signal determination on the PCR product. By the controllable modification of the primer coupling quantity on gold nanoparticle surface, in order to achieve the stability of gold nanoparticles in PCR system and carry out modification on the nanoparticle-primer conjugates,gold dimer is assembled and comparison of TEM character and CD signal is performed in a suitable PCR condition. Except for the assembly of gold nanoparticles with same particle size, the invention also studies the assembly of gold nanoparticles with different particle sizes and their properties.

Description

technical field [0001] A polymerase chain reaction-based research on the controllable assembly of gold dimers and their chiral properties belongs to the field of material chemistry. Background technique [0002] Nanomaterials refer to materials with at least one dimension in the nanometer scale (1-100nm) in three-dimensional space, or materials composed of them as basic units, which is approximately equivalent to the scale of 10-100 atoms closely arranged together. It is a new generation of materials composed of nanoparticles whose size is between atoms, molecules and macroscopic systems. Since the size of nanomaterials is close to the de Broglie wavelength of electrons, the superconducting coherence wavelength, and the Bohr radius of excitons, electrons are confined to a very small space, and their wave functions are limited. In addition, they have a large surface area. Therefore, nanomaterials show novel optical, electrical, magnetic and chemical properties different from...

Claims

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Application Information

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IPC IPC(8): C12N15/10B22F9/24B82Y40/00
Inventor 王利兵胥传来赵媛
Owner 王利兵
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