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Tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge

A technology of tissue culture and Salvia miltiorrhiza, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of virus-carrying and low bacterial infection rate in seedlings, and achieves overcoming browning and high transplanting survival rate. , the effect of high bud induction rate

Inactive Publication Date: 2012-05-02
SICHUAN AGRI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the rapid propagation of Chuandanshen No. 1 tissue culture for the long-term use of root propagation and virus-carrying seedlings in the breeding of No. and Danshen tissue culture seedlings with low infection rate

Method used

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Embodiment 1

[0024] In December 2010, the roots of Chuandanshen No. 1 were selected for cutting propagation. After the new shoots grew in the spring of 2011, tissue culture and rapid propagation were carried out. The specific methods are as follows:

[0025] 1. Select the explant: cut off the tender stem section 3cm with the terminal bud and the band joint from the No. 1 plant of Salvia miltiorrhiza in Sichuan to be the explant.

[0026] 2. Disinfection treatment: wash the material with tap water, wash with washing powder, rinse with running water for 30 minutes, rinse twice with sterile water on the ultra-clean workbench, soak in 75% alcohol for 30 seconds, rinse with sterile water for 3 times, and then rinse with 0.1% alcohol HgCl2 and dropwise added 3 drops of Tween-80 for disinfection for 6 minutes, and then rinsed with sterile water for 6 times;

[0027] 3. Induction culture: cut 1.0 cm young stems of Salvia miltiorrhiza with terminal buds or joints from the sterilized materials with ...

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Abstract

The invention provides a tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge, wherein in the method, a tender stem section is utilized as an explant. The method comprises the following steps: selecting the explant; disinfecting the explant; carrying out induction culture; carrying out multiplication culture, carrying out rooting culture; and carrying out acclimatization and transplant. According to the tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge, a tissue culture technology is productively applied to the rapid propagation of Szechuan salvia miltiorrhiza bunge, and a culture method combining bud induction and bud mulitiplication is adopted, thus the browning of stem section tissues of Szechuan salvia miltiorrhiza bunge is effectively overcome and the browning is reduced by 20%. The tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge has the advantages of high bud induction rate and high transplant survival rate, wherein the bud induction rate can generally reach above 85% and the transplant survival rate can generally reach above 80%.

Description

technical field [0001] The invention relates to a tissue culture method, more specifically to a tissue culture rapid propagation method of Chuandanshen No. 1. technical background [0002] Salvia miltiorrhiza Bunge is a perennial herb of the genus Salvia in the family Lamiaceae. Its roots and rhizomes are used as medicine. . It was first recorded in "Shen Nong's Materia Medica", listed as the top grade, and is the only source of the traditional Chinese medicine Salvia miltiorrhiza in the previous editions of "The Pharmacopoeia of the People's Republic of China". It is slightly cold in nature, bitter in taste, non-toxic, and has the effects of promoting blood circulation and promoting menstruation, removing blood stasis and relieving pain, clearing heart and calming nerves. It is used to treat irregular menstruation, amenorrhea, dysmenorrhea, accumulation of addicted lumps, tingling pain in the chest and abdomen, heat pain, swelling and pain of sores, upset and insomnia, he...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张利倪苏谢显莉王萌邹章满周永红杨瑞武雷淑琼
Owner SICHUAN AGRI UNIV
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