Method for creating common maize inbred-line by introducing teosintes in maize cultivated specie

A technology for maize cultivation and ruminant grass, which is applied in the directions of botanical equipment and methods, application, and plant genetic improvement, and achieves the effects of high selection efficiency, simple application program, and easy mastery and implementation.

Inactive Publication Date: 2012-06-06
重庆三峡农业科学院
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Problems solved by technology

Therefore, artificial environme

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  • Method for creating common maize inbred-line by introducing teosintes in maize cultivated specie
  • Method for creating common maize inbred-line by introducing teosintes in maize cultivated specie
  • Method for creating common maize inbred-line by introducing teosintes in maize cultivated specie

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Embodiment Construction

[0035] The current examples further illustrate the method of introducing teosinte into maize cultivars to create common maize inbred lines.

[0036] In the spring of 2006, pot experiments were adopted in our hospital (located at 30°37′56″N, 760 meters above sea level), and Mexican annual diploid maize (teosinte) was sown in late March, and passed through an artificial darkroom from emergence Short-day treatment (12 hours of fixed light per day) was carried out to induce the Mexican annual diploid maize (teosinte) to flower around June 20. While sowing the Mexican annual diploid maize (teosinte), The inbred lines A801, 286-4, XZ966-14, L-41, WSh, etc. of maize cultivars intended to be used as recipients were sown separately, and then sowed every 10 days for one period, and each period contained 20 seedlings per line. Results No. One, 5 corn cultivars inbred lines of the second phase sowing all meet well with Mexico's annual diploid class corn (teosinte) flowering period.Adopt t...

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Abstract

The invention discloses a method for creating a common maize inbred-line by introducing teosintes in a maize cultivated specie, belonging to a method for creating maize breeding materials in crop breeding. The method integrates multiple technologies of creating the common maize inbred line, comprising the following steps: step 1, environment variation: giving only 12 hours of illumination everyday in an artificial darkroom to let teosintes and the maize cultivated specie flower in the same period; step 2, introduction of hybridization: according to a routine hybridization bagging method, using the cultivated specie as the female parent and the teosintes as the male parent to hybridize; step 3, backcrossing improvement: using the female parent cultivated specie corresponding to F1 as the female parent, taking F1 mixed pollen to backcross with the female parent to obtain BC1 seeds, sowing the BC1 seeds, taking BC1 mixed pollen to carry out secondary backcross with the female parent to obtain backcrossing second generation (BC2) seeds; step 4, pedigree breeding: sowing the BC2 seeds, preferably individual plants, carrying out continuous inbreeding of more generations to obtain a stable inbred-line; and step 5, using the stable inbred-line as the female parent, taking test line pollen to hybridize, planting and identifying the hybridized combinations, and comparing and screening excellent combinations.

Description

technical field [0001] "Method of introducing teosinte into maize cultivars to create common maize inbred lines" belongs to agronomy (code: 210) - agronomy (code: 210.30) - crop breeding and improved seed breeding (code: 210.3035) in maize Breeding material creation method. Background technique [0002] Corn is one of the three major grain crops (rice, wheat, corn) in China, with a perennial cultivated area of ​​nearly 400 million mu, and its total and per unit yield ranks second among grain crops; corn is also the main livestock feed and important food , medicine, and industrial raw materials, therefore, corn plays a pivotal role in my country's national economic and social development. In the past 30 years, the level of corn technology in my country has been significantly improved, and the yield per unit area and total yield have nearly doubled, among which the genetic gain due to variety improvement has played a leading role. [0003] The characteristics of maize variety...

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Application Information

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IPC IPC(8): A01H1/02
Inventor 霍仕平张兴端向振凡张健晏庆九余志江张芳魁
Owner 重庆三峡农业科学院
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