Pure draft beer film cleaning and regenerating enzyme preparation and cleaning method using same

A technology for regenerating enzymes and enzyme preparations, applied in the field of fermentation engineering, can solve problems such as membrane fouling, ultrafiltration membrane clogging, and reduced membrane processing capacity, and achieve the effects of saving use costs, prolonging service life, and improving regeneration and recovery capabilities

Inactive Publication Date: 2012-06-13
GUANGXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent CN 100503818C has invented a kind of ultrafiltration membrane cleaning enzyme used in the fruit and vegetable juice ultrafiltration concentration process and a production method for improving the flux of the ultrafiltration membrane. , hemicellulose, protein, etc., pectinase, cellulase, hemicellulase, protease and other components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of Cleaning and Regenerating Enzyme and Membrane Cleaning in the Production of a Pure Draft Beer

[0049] Composition of raw materials: cleaning regeneration enzyme I, cleaning regeneration enzyme II.

[0050] Wash and regenerate enzyme I:

[0051] Pentosanase 375g, β-glucanase 100g, mannanase 25g.

[0052] Preparation method: mechanically mix pentosanase, β-glucanase, and mannanase according to the above proportions in a sterile environment.

[0053] Wash Regenase II:

[0054] Proline endoprotease 250g, neutral protease 250g.

[0055] Preparation method: mix the proline endoprotease and neutral protease according to the above ratio in a sterile environment.

[0056] Membrane cleaning:

[0057] A set of microporous filter membranes (0.45μm, 750mm, 36 pieces) filters pure draft beer according to production requirements, and then regenerates according to conventional acid-base cleaning methods: prepare 55°C hot water in the lye tank, pour it into the memb...

Embodiment 2

[0068] Preparation of cleaning regeneration enzyme and membrane cleaning in another pure draft beer production

[0069] Composition of raw materials: cleaning regeneration enzyme I, cleaning regeneration enzyme II.

[0070] Wash and regenerate enzyme I:

[0071] Pentosanase 375g, β-glucanase 200g, mannanase 25g.

[0072] Preparation method: mechanically mix pentosanase, β-glucanase, and mannanase according to the above proportions in a sterile environment.

[0073] Wash Regenase II:

[0074] Proline endoprotease 300g, neutral protease 200g.

[0075] Preparation method: mix the proline endoprotease and neutral protease according to the above ratio in a sterile environment.

[0076] Production test:

[0077] A set of microporous filter membranes (0.45μm, 750mm, 36 pieces) filters pure draft beer according to production requirements, and then regenerates according to conventional acid-base cleaning methods: prepare 55°C hot water in the lye tank, pour it into the membrane fi...

Embodiment 3

[0088] Preparation of cleaning regeneration enzyme and membrane cleaning in another pure draft beer production

[0089] Composition of raw materials: cleaning regeneration enzyme I, cleaning regeneration enzyme II.

[0090] Wash and regenerate enzyme I:

[0091] Pentosanase 315g, β-glucanase 315g, mannanase 70g.

[0092] Preparation method: mechanically mix pentosanase, β-glucanase, and mannanase according to the above proportions in a sterile environment.

[0093] Wash Regenase II:

[0094] Proline endoprotease 375g, neutral protease 125g.

[0095]Preparation method: mix the proline endoprotease and neutral protease according to the above ratio in a sterile environment.

[0096] Production test:

[0097] A set of microporous filter membranes (0.45μm, 750mm, 36 pieces) filters pure draft beer according to production requirements, and then regenerates according to conventional acid-base cleaning methods: prepare 55°C hot water in the lye tank, pour it into the membrane fil...

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PUM

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Abstract

The invention discloses a pure draft beer film cleaning and regenerating enzyme preparation and a cleaning method using the same. The film cleaning and regenerating enzyme preparation designed according to the pure draft beer film plug substance composition comprises the following components: cleaning and regenerating enzyme I: 40-80% of pentosan enzyme (10000U/g), 10-50% of beta-glucanase (20000U/g) and 2-20% of mannase (10000U/g); and cleaning and regenerating enzyme II: 40-80% of proline endoproteinase (5U/g) and 20-60% of neutral proteinase (100000U/g). The optimized pure draft beer film cleaning and regenerating method comprises the following steps: conventional water washing and alkaline-process cleaning; cyclic cleaning by an enzyme process while keeping the temperature; impregnated cleaning by an enzyme process; water washing; alkaline-process cleaning; and integrity testing. The film cleaning and regenerating enzyme disclosed by the invention effectively solves the problem of film flux depression caused by the pure draft beer film plug substance, and enhances the film regeneration power and pure draft beer filtration capacity by more than 35%.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, relates to the beer fermentation production industry, in particular to a pure draft beer membrane cleaning regeneration enzyme preparation and a cleaning method. Background technique [0002] In the production of pure draft beer, the low-temperature microporous membrane filtration sterilization process is used to replace the traditional pasteurization process, which makes the beer taste fresher, purer and more nutritious, and is favored by consumers. The key to pure draft beer production technology is aseptic brewing, aseptic filtration and aseptic filling, and microporous membrane filtration technology is the core of pure draft beer production. Membrane filtration system for pure draft beer. At present, breweries at home and abroad generally use two sets of filtration systems, coarse and final, to filter sake. Using its fine physical filtration characteristics (coarse filtration 0.65 μm, ...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N9/24C12N9/42C12N9/50B08B7/04B08B3/08B08B3/04
Inventor 蒙健宗郑翔鹏李晓明梁树华马少敏张云光韦函忠
Owner GUANGXI UNIV
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