Detection kit and preparation method thereof for drugs of benzodiazepines
A detection kit and a technology of benzodiazepines, which are applied in the field of immunocolloidal gold rapid detection kit and its preparation, can solve the problems of slow speed and complicated operation, and achieve the advantages of reduced dosage, sensitive color reaction and stable effect Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0064] The preparation of the detection kit of embodiment 1 benzodiazepines
[0065] Reagent source:
[0066] Anti-benzodiazepine monoclonal antibody, goat anti-mouse IgG polyclonal antibody, benzodiazepine-bovine serum albumin complex (BZ0-BSA): purchased from MAXMED LABORATORIES INC.
[0067] Nitrocellulose film, glass fiber paper: purchased from Germany Sartorius (SARTORIUS)
[0068] Reagent preparation:
[0069] 0.1M pH7.6 phosphate buffer: weigh 10.2gNa 2 HPO 4 , 3.4gNaH 2 PO 4 , add 900ml of purified water to dissolve, adjust the pH value of the solution to 7.6 with hydrochloric acid, and dilute to 1000ml with purified water.
[0070] 10% bovine serum albumin (BSA) solution: Weigh 100 g of BSA, dissolve it in purified water, and dilute to 1000 ml.
[0071] 0.1% BSA borate buffer solution: Weigh 9.535g of borax and dissolve it in 900ml of purified water, adjust the pH value to 8.6±0.05 with hydrochloric acid, and set the volume to 1000ml to obtain 0.1M borate buffe...
Embodiment 2
[0109] The sensitivity experiment of the detection kit of embodiment 2 benzodiazepines
[0110] Detection method:
[0111] 1. Take the detection kit of benzodiazepines prepared in Example 1, and place the kit on a horizontal platform.
[0112] 2. Take the sample with the sample pipette, and then drop 3 drops (about 120ul) of the sample into the sample hole of the kit. Use a different pipette for each different sample.
[0113] 3. Observation results: Interpret the results 3-8 minutes after adding the sample.
[0114] Test sample:
[0115] Quality control reference substances with oxazepam concentrations of 150ng / ml, 300ng / ml, and 600ng / ml were prepared as samples, and each concentration of each kit was repeated 6 times.
[0116] Sensitivity test result table
[0117] ng / ml
[0118] Oxazepam concentration
[0119] The detection sensitivity results of benzodiazepines
[0120] ng / ml
[0121] Element
[0122] The results confirmed that the prepared det...
Embodiment 3
[0123] The specificity experiment of the detection kit of embodiment 3 benzodiazepines
[0124] Using the method of Example 2, the following drugs were detected to observe whether cross-reaction occurred.
[0125] cross test
[0126]
[0127]
[0128] The test results show that the above drugs will not affect the test results when the concentration is equal to 100 μg / ml.
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
particle size | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com